Plant/Algal cell antibodies / Populus sp.

product information

TIP2-1 belongs to aquaporin protein family and facilitates transport of water and small neutral solutes across tonoplast membrane.

KLH-conjugated peptide derived from N-terminus of Oryza sativa TIP2-2 Q5Z6F0

store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.

ELISA (ELISA), western blot (WB)

collection of antibodies to tonoplast proteins

AS09 492 | anti-TIP1;1, TIP1;2 | tonoplast intrinistic protein 1-1, 1-2 (gamma)

AS09 493 | anti-TIP1;1, TIP1;2 | tonoplast intrinistic protein 1-1, 1-2 (gamma)

AS09 494 | anti-TIP2;1 (delta) | Delta-VM23

AS09 510 | anti-TIP2;1 | tonoplast intrinistic protein 2-1

AS09 511 | anti-TIP2;2 | tonoplast intrinistic protein 2-2 (C-terminal)

0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.

Antibodies will detect target protein in a few  µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.

Vacuolar membrane protocol is available here.

application information

1: 8000 (ELISA), 1: 2000 with standard ECL (WB)

25 | 20-24 kDa

Oryza sativa

dicots including: Arabidopsis thaliana, Ricinus communis, Spinacia oleracea, Vitis vinifera, monocots including: Hordeum vulgare, Triticum aestivum, Zea mays, trees: Populus trichocarpa

no confirmed exceptions from predicted reactivity known in the moment

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.

Diluted antibody solution can be used 2 to 3 times within one month if it  contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.

Manufactured by Operon Biotechnologies.

Sakurai et al. (2008). Tissue and cell-specific localization of rice aquaporins and their water transport activities- Plant Cell Physiol. 49: 30-39.