COXII | cytochrome oxidase subunit II

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Cytochrome c oxidase (COX) catalyzes the reduction of oxygen to water in the respiratory chain in the inner mitochondrial membrane. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 (COXII) transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1. Alternative name: cytochrome c oxidase subunit 2

immunogen

KLH-conugated synthetic peptide fully conserved in all available protein sequences from eudicots includingArabidopsis thaliana AtmG00160, monocots including Oryza sativa P04373 and Physcomitrella patens Q1XGA9

antibody format

rabbit

polyclonal

affinity purified serum, in PBS pH 7.4

lyophilized

quantity

100 µg

for reconstitution add 100 µl of sterile water.

storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

tested applications

western blot (WB), Blue Native-PAGE (BN-PAGE)

related products

AS04 052 anti-COXII hen antibody

AS04 151 ant-COXIIb rabbit antibody

antibodies to other mitochondrial proteins

recommended secondary antibody

additional information

cellular [compartment marker] of mitochondrial inner membrane

application information
recommended dilution		1:1000 (WB), 1: 1000 (BN-PAGE)
expected \| apparent MW		29.4 \| 30 kDa (for Arabidopsis thaliana)
confirmed reactivity		Arabidopsis thaliana (leaf extract and isolated mitochondria), Betula nana, Brassica oleracea, Cicer aretinum, Erophorum vaginatum, Lilium longifolorum, Plantago major, Plantago euryphylla, Silene uniflora, Silene dioica, Physcomitrella patens, Tritirum durum Desf. , Zea mays, Quercus rubra
predicted reactivity		dicots including Cucumis melo, Glycine max, Nicotiana tabacum, Pisum sativum, monocots including Oryza sativa, moss Physcomitrella patens
not reactive in		no confirmed exceptions from predicted reactivity known in the moment
additional information		antibody detects COXII protein in total leaf extracts and isolated mitochondria Blue Native gel electrophoresis (BN-PAGE) has been performed on samples solubilized with digitonin (4:1) and loaded at 100 µg/well. Gel thickness was 2 mm with 4.5-16 % gradient.
selected references		Subba et al. (2013). Characterization of the nuclear proteome of a dehydration-sensitive cultivar of chickpea and comparative proteomic analysis with a tolerant cultivar. Proteomics, DOI: 10.1002/pmic.201200380. Hopff et al. (2013). The plasma membrane proteome of maize roots grown under low and high iron conditions. J Proteomics Jan 24. Lang, E.G.E., S.J. Mueller, S.N.W. Hoernstein, J. Porankiewicz-Asplund, M. Vervliet-Scheebaum, R. Reski (2010). Simultaneous isolation of pure and intact chloroplasts and mitochondria from moss as basis for sub-cellular proteomics. Plant Cell Reports, DOI: 10.1007/s00299-010-0935-4. (open source).

application example

1.8 µg of total protein from (1) Arabidopsis thaliana leaf , (2) Plantago major leaf, (3) Plantago europhyllaleaf, (4) Silene dioica leaf, (5) Silene uniflora leaf were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Primary antibody was used in 1: 1000 dilution.

western blot detection using anti-COXII antibody

anti-plant COXII antibody in BN-PAGE

BN-PAGE/SDS-PAGE analysis usinf anti-COXII antibodies, which confirms that COXII is the only protein of a complex IV which is recognized by anti-COXII antibodies.

Courtesy Dr. Janusz Piechota, Wroclaw University, Poland

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