|
product information
|
| background |
|
Cytochrome c is located in inner mitochondrial membrane. It is a small heme protein which, unlike other cytochromes, is highly soluble. This protein is an essential component of the electron transport chain, where it undergoes oxidation and reduction without binding oxygen. |
| immunogen |
|
synthetic peptide derived from Arabidopsis thaliana cytochrome c protein sequence, accession At1g22840 (Cytc1) and At4g10040 (Cytc2) coupled to KLH |
| antibody format |
|
rabbit; |
polyclonal; |
|
serum; |
lyophilized |
|
| quantity |
|
200 µl |
- for reconstitution add 200 µl of sterile water |
|
| storage |
|
store lyophilized at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles and store at -80°C. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| tested applications |
|
Western blot (WB) |
| related products |
|
AS04 052 | anti-COXII hen antibody AS04 053A | anti-COXII rabbit antibody AS06 151 | anti-COXIIb antibody |
| additional information |
|
RECONSTITUTED ANTIBODY NEEDS TO BE STORED AT -80°C. |
application information
|
| recommended dilution |
|
1: 1000 with standard ECL (WB) |
| expected | apparent MW |
|
12,5 | 14 kDa for A. thaliana |
| confirmed reactivity |
|
Arabidopsis thaliana, Lupinus luteus
|
| predicted reactivity |
|
cytc1 and cytc2 from following species: A. theoprasi, B. napus, B. oleracea, C. maxima, Ch. reinhardtii (peptide target partially conserved), L. esculentum ,M. truncatula, O. sativa, Ostreococcus (peptide target partially conserved), P. aurea, Physcomitrella patens, R. comunis, S. nigra, V. vinifera |
| not reactive in |
|
Arabidopsis thaliana CytC6 |
| additional information |
|
The presence of cytochrome c in the cysotol is a marker of PCD (programmed cell death). |
| selected references |
|
to be added when available |
application example 
The presence of cytochrome C (Cyt c) in the cytosol is a marker of plant Programmed Cell Death (PCD). During PCD, cytochrome C may be released from the mitochondrial intermembrane space and the VDAC1 protein stays associated with mitochondria. After PCD induction, Arabidopsis protoplasts were gently disrupted using a 100 μm mesh to keep mitochondria intact and the total extract was fractionated at 15 000 g into a cytosolic fraction and a pellet containing mitochondria. Proteins of each fraction was blotted onto a PVDF membrane for 2 hours at 50 v/cm. The membrane was blocked with 3% BSA in PBS-T for 1 hour and then incubated with Agrisera cytochrome C antibody (1:1500) for I h at RT, followed by 3 PBST washes and incubation with Agrisera goat-anti-rabbit IgG HRP (AS09 602) at 1:50 000 for one h at RT. After 3 PBST wash and addition of the ECL substrate (Pierce pico: fento 4;1), the blot was exposed one minute. After striping, the blot was reprobed using Agrisera VDAC1 antibody (1:3000) in TBST . Courtesy Dr. Patrick Gallois, University of Menchester, UK
||| For applications or usage on species others than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use LiveChat option in the left menu or contact us at support@agrisera.com
 Save as PDF
|
