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product information
|
| background |
|
FtsH belong to a family of ATP dependent peptidases.
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| immunogen |
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KLH-conjugated peptide dereived from sequences of all known FtsH isoforms of Arabidopsis thaliana including FtsH3 and FtsH10 with localization to mitochondria Q84WU8 and Q8VZI8 |
| antibody format |
|
rabbit |
polyclonal |
|
serum |
lyophilized |
|
| quantity |
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500 µl |
for reconstitution add 500 µl of sterile water. |
|
| storage |
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store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| tested applications |
|
western blot (WB) |
| related products |
|
AS07 205 | anti-FtsH4 | plant fts metallo-protease H4 AS07 251 | anti-FtsH10 | plant fts metallo-protease H10 |
| additional information |
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to be added when available |
application information
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| recommended dilution |
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1 : 500- 1: 1000 with standard ECL (WB) |
| expected | apparent MW |
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depends upon detected isoform |
| confirmed reactivity |
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Arabidopsis thaliana, Hordeum vulgare, Zea mays
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| predicted reactivity |
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di and monocots
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| not reactive in |
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no confirmed exceptions from predicted reactivity known in the moment |
| additional information |
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to be added when available |
| selected references |
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to be added when available, antibody released in October 2011. |
application example 
8 µg of total protein from Arabidopsis thaliana wild type (1) and FtsH 1 and 3 deletion mutant (2) extracted with 0.2M Tris-HCl pH6.8, 2%SDS, 10% mercaptoehanol, 5M Urea were separated on 12 % SDS-PAGE and blotted 2h to NC. Blots were blocked with non fat milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2 000 over night at 4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 5 min and 2 times for 10 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody anti-rabbit IgG horse radish peroxidase conjugated, from Sigma) diluted to 1:10 000 in TBS-T+5% non fat milk for 2h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturers instructions. Exposure time was 3 minutes. Courtesy of Dr. Leah Nave, The Hebrew University, Israel
||| For applications or usage on species others than stated as confirmed above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menue bar or contact us at support@agrisera.com
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