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product information
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| background |
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Galactono-1,4-lactone dehydrogenase (GLDH) is the enzyme which catalyses last step of ascorbic acid (AA) synthesis in the mitochondria of plant cells. Synonyme: GaLDH |
| immunogen |
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recombinant C-terminal of maize GLDH |
| antibody format |
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rabbit |
polyclonal |
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total IgG in PBS pH 7.4 |
lyophilized |
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| quantity |
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100 µl |
for reconstitution add 100 µl of sterile water. |
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| storage |
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store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| tested applications |
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western blot (WB) |
| related products |
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collection of antibodies to stress proteins
collection of antibodies to mitochondrial proteins |
| additional information |
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total IgG concentration is 6.8 ug/ul |
application information
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| recommended dilution |
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1: 5000 with standard ECL (WB) |
| expected | apparent MW |
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68 kDa |
| confirmed reactivity |
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Avena sativa,Glycine max, Hordeum vulgare,Helianthus annuus, Zea mays |
| predicted reactivity |
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n.a. |
| not reactive in |
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no confirmed exceptions from predicted reactivity known in the moment |
| additional information |
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mitochondrial, membrane or meristematic fractions were shown to be richer in GLDH expression |
| selected references |
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Bartoli et al. (2005). Ascorbate content in wheat leaves is not determined by maximal L-galactono-1, 4-lactone dehydrogenase (GalLDH) activity under drought stress. Plant Cell Environ 28:1073-1081. |
||| For applications or usage on species others than stated as confirmed above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menue bar or contact us at support@agrisera.com
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