Plant/Algal cell antibodies / Mitochondria | Respiration

product information

The Glycine decarboxylase complex (GDC) is abundant in mitochondria of C3 leaves and functions in photorespiratory carbon recovery. GDC  enzyme can account for up to 50% of matrix protein, and is responsible for the most prominent metabolic activity in the mitochondria of illuminated leaves, photorespiration. GDC is a multienzyme complex composed of four component enzymes, the P-, H-, T-, and L-proteins and is responsible for the conversion of glycine produced in the peroxisome to serine in the mitochondria during photorespiratory cycle. The H-protein plays a key role as a mobile substrate that commutes between the other subunits, allowing its lipoic acid “arm” to visit the active sites of the other three components.

purified GDC-H protein from Spinacia oleracea

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

western blot (WB)

AS06 203A | anti- Idh | isocytrate dehydrogenase rabbit antibody, marker of mitochondrial matrix

AS07 212 | anti-VDAC1 marker antibody for mitochondrial outer membrane

cellular [compartment marker] of mitochondrial matrix

application information

1:5 000 with standard ECL (WB)

16 kDa

Arabidopsis thalina, Spinacia oleracea

n.a.

no confirmed exceptions from predicted reactivity known in the moment

this antibody can be used on total cell extract of Arabidopsis thaliana

application example

15 µg of total protein from  Arabidopsis thaliana leaf extract has been loaded per lane. Primary antibody has been used in 1: 5000 dilution using standard ECL.