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product information
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| background |
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Nucleoside diphosphate kinase protein (EC=2.7.4.6) is catalysing the transfer of a g-phosphate group from adenosine triphosphate (ATP) to a cognate nucleoside diphosphate. This contributes to balancing of the nucleoside pool. NDPK enzymes are present in most subcellular compartments of the eukaryotic cell. |
| immunogen |
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KLH-conjugated synthetic peptide derived from Pisum sativum NDPK Q9SP13 |
| antibody format |
|
rabbit |
polyclonal |
|
serum |
lyophilized |
|
| quantity |
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200 µl |
for reconstitution add 200 µl of sterile water. |
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| storage |
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store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| tested applications |
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western blot (WB) |
| related products |
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peptide used for immunization is also conserved in NDPK3 of Arabidopsis thaliana O49203 |
| additional information |
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to be added when available |
application information
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| recommended dilution |
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1: 5000 (WB) |
| expected | apparent MW |
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25 kDa |
| confirmed reactivity |
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Arabidopsis thaliana, Pisum sativum |
| predicted reactivity |
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dicots including: Brassica campestris, Spinacia oleracea, Vitis vinifera, monocots indlucing: Oryza sativa |
| not reactive in |
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no confirmed exceptions from predicted reactivity known in the moment |
| additional information |
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1: 5000 dilution in a western blot was used for 15 µg of protein per lane |
| selected references |
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Hammargren et al. (2007). On the phylogeny, expression and targeting of plant nucleoside diphosphate kinases. Physiol Plant. 129: 79–89. |
||| For applications or usage on species others than stated as confirmed above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menue bar or contact us at support@agrisera.com
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