SHMT | serine hydroxymethyltransferase

320 €

AS05 075 | clonality: polyclonal | host: rabbit | reactivity: A.thaliana, P. sativum, S. oleracea | cellular [compartment marker] of mitochondrial matrix

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product information

background

Serine hydroxymethyltransferase (SHMT) is part of the mitochondrial enzyme complex.

immunogen

purified SHMT protein from Spinacia oleracea

antibody format

rabbit

polyclonal

total IgG in PBS pH 7.4

lyophilized

quantity

200 µl

for reconstitution add 200 µl of sterile water.

storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

tested applications

western blot (WB)

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additional information

to be added when available

application information
recommended dilution		1:5 000 with standard ECL (WB)
expected \| apparent MW		53 kDa (Arabidopsis thaliana)
confirmed reactivity		Arabidopsis thaliana, Pisum sativum, CSpinacia oleracea
predicted reactivity		dicots including: Glycine max, Medicago truncatula, Ricinus communis, Solanum tuberosum, Vitis vinifera, monocots: Hordeum vulgare, trees: Populus balsamifera
not reactive in		no confirmed exceptions from predicted reactivity known in the moment
additional information		this antibody can be used on total leaf extracts and isolated mitochondria
selected references		Camejo et al. (2012). Salinity-induced changes in S-nitrosylation of pea mitochondrial proteins. J. Proteomics Dec.11.

application example

total leaf extract from Arabidopsis thaliana has been loaded per lane based on a leaf disc area. Primary antibody has been used in dilution 1: 5000 following detection using standard ECL. c

western blot detection using anti-SHMT antibody

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