Plant/Algal cell antibodies / Secondary antibodies/blocking / Anti-goat

product information

Rabbit anti-goat IgG is a secondary antibody conjugated to HRP (horse radish peroxidase) which binds to all goat immunoglobulins in immunological assays.

Undiluted antibody conjugate is stable for 6-8 weeks at 2-8°C. For long term storage, dilute with 50% glycerol and store at -20°C as a liquid. Prepare working dilution prior to use and then discard.

AS09 612 | Rabbit anti-goat IgG (H&L) biotin conjugated

1: 10 000 -1: 50 000 (ELISA),  1: 10 000 - 50 000 with regular ECL (WB), 1: 500 -1: 5000 (IHC)

HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free

0.1 % (v/v) of Kathon CG is used as preservative.

No reactivity is observed to non-immunoglobulin goat  serum proteins based in immunoelectrophoresis

application example

5 µg of total protein from A.thaliana (1), H. vulgare (2),  Z.mays (3),  S. oleracea (4), extracted with Agrisera PEB extraction buffer (AS08 300) were separated on  4-12% SDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following transfer in  for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 10 000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-goat IgG horse radish peroxidase conjugated, from  Agrisera AS09 605) diluted to 1:50 000  for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with ECL  detection reagent according to the manufacturers instructions.  Exposure time was 5 seconds.