Plant/Algal cell antibodies / Secondary antibodies/blocking / Anti-chicken

product information

Goat anti-hen IgY is a secondary antibody conjugated to HRP (horse radish peroxidase) which binds to hen IgY in immunological assays.

Undiluted antibody conjugate is stable for 6-8 weeks at 2-8°C. For long term storage, dilute with 50% glycerol and store at -20°C as a liquid. Prepare working dilution prior to use and then discard.

AS09 606 | Goat anti-hen IgY (H&L) AP conjugated

AS09 610 | Goat anti-hen IgY (H&L) biotin conjugated

1: 10 000 -1: 150 000 (ELISA), 1 : 20 000 with ECL Advance and 1: 10 000 with regular ECL (WB), 1: 500 -1: 5000 (IHC)

Antibody concentration is 1 mg/ml

HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free

0.1 % (v/v) of Kathon CG is used as preservative.

No reactivity is observed to non-immunoglobulin hen serum proteins based in immunoelectrophoresis

Hen immunoglobulin is often called hen or chicken IgG

application example

5 µg of total extract from (1) Hordeum vulgare total leaf,  (2) Zea mays  (3) Spinacia oleracea extracted with PEB (AS08 300) were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following transfer in 2% ECL Advance blocking reagent (GE Healthcare) in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary anti-PsbA antibody (AS01 016) at a dilution of 1: 10 000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-hen IgG horse radish peroxidase conjugated, AGRISERA) diluted to 1:50 000 in 2% ECL Advance blocking solution for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with ECL Advance detection reagent according to the manufacturers instructions. Images of the blots were obtained using a CCD imager (FluorSMax, Bio-Rad) and Quantity One software (Bio-Rad). Exposure time was 30 seconds.