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Cyt f | cytochrome f protein (PetA) of thylakoid Cyt b6/f-complex

320 €

AS08 306 | clonality: polyclonal | host: rabbit | reactiviy: Arabidopsis thaliana, Thermosynechococcus elongatus, Zea mays

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AS08 306

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product information
background  

Multi-subunit complex of cytb6/f is a crucial component for the photosynthetic electron transport chain of higher plants, green algae and cyanobacteria. This complex is catalyzing oxidation of quinols and the reduction the reduction of plastocyanin. This reaction allows to establish the proton force required for the ATP synthesis. Four major subunits build the complex: the petA gene product corresponding to a c-type cytochrome (cytf), the petB gene product corresponding to a b-type/c’-type cytochrome with three haems (cyt b6), the petD gene product (subunit IV, or suIV), and the petC gene product, corresponding to the Rieske/Iron/sulfur protein.

immunogen  

maize cytochrome f purified from chloroplasts, including a final gel purification on a denaturing gel. protein used to elicit this antibody is conserved in Arabidopsis thaliana cyt f P56771, AtCg00540

antibody format  

rabbit

polyclonal

serum

lyophilized

quantity  

100 µl

for reconstitution add 100 µl of sterile water.

storage  

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

tested applications  

western blot (WB)

related products  

AS06 119 | anti-cytochrome f (PetA) rabbit antibody

AS07 231 | anti-cytochrome f (PetA) rabbit antibody

additional information  
application information
recommended dilution  

1: 2500- 1:5000 with standard ECL (WB)

expected | apparent MW  

31-32 kDa

confirmed reactivity  

Arabidopsis thaliana, Thermosynechococcus elongatus, Zea mays

predicted reactivity  

dicots including: Nicotiana tabacum, Solanum tuberosum, monocots including: Hordeum vulgare, Oryza sativa, trees: Populus trichocarpa

not reactive in  

no confirmed exceptions from predicted reactivity known in the moment

additional information  

not available at the moment

selected references  

Asakura & Barkan (2007). A CRM domain protein functions dually in group I and group II intron splicing in land plant chloroplasts.Plant Cell 12:3864-3815



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