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Gamma-ECS | gamma glutamylcysteine synthase

320 €

AS06 186  | clonality: polyclonal  |  host: rabbit  |  reactivity: N.tabacum, S. lycopersicum, Z. mays

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AS06 186

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product information
background  

Gamma-glutamylcysteine synthase (gamma-ECS) (EC 6.3.2.2) catalyses the first step of glutathione (GSH) synthesis, producing gamma-glutamylcysteine (gamma-EC) from L-glutamate and cysteine. In the second step, catalyzed by GSH-S, glycine is added to the C-terminal end of (gamma-EC). GSH is the predominant non-protein thiol in all nearly where it acts as an antioxidant in stress responses and as a mobile pool of reduced sulfur. It is also important in the regulation of plant growth and development.

immunogen  

KLH-conjugated synthetic peptide derived from Zea mays  gamma-ECS Q8W4W3

antibody format  

rabbit

polyclonal

total IgG in PBS pH 7.4

lyophilized

quantity  

100 µl

for reconstitution add 100 µl of sterile water.

storage  

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

tested applications  

western blot (WB), immunolocalization (IL)

related products  

collection of antibodies to plant stress proteins

additional information  

total IgG concentration is 5.2 µg/ µl

application information
recommended dilution  

1: 5000 with standard ECL (WB), 1: 5000  (IL)

expected | apparent MW  

50 kDa

confirmed reactivity  

Nicotiana tabcum, Solanum lycopersicum, Zea mays

predicted reactivity  

dicots including:Arabidopsis thalina, Pisum sativum, Riciuns communis, monocots including: Oryza sativa, Triticum aestivum

not reactive in  

no confirmed exceptions from predicted reactivity known in the moment

additional information  

antibodies has been used in immunolocalization on Arabidopsis thaliana

selected references  

Ghanta et al. (2011). Nicotiana tabacum overexpressing γ-ECS exhibits biotic stress tolerance likely through NPR1-dependent salicylic acid-mediated pathway. Planta 233(5):895-910.

Gomez et al. (2004). Intercellular distribution of glutathione synthesis in maize leaves and its response to short term chilling. Plant Physiol 134: 1662-1671; Mittova et al. (2003). Co-ordinate induction of glutathione biosynthesis and glutathione-metabolising enzymes is correlated with salt tolerance in tomato. FEBS Letts. 554: 417-421.



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