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H3K36me3 | Histone H3 trimethylated lysine 36 (ChIP grade)
Product no: AS16 3196
AS16 3196 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A.thaliana, Human, Mouse, O. sativa
533
€
Delivery:
3-6 business days
- Product Info
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Immunogen: KLH-conjugated synthetic peptide Host: Rabbit Clonality: Polyclonal Purity: Immunogen affinity purified serum. Format: Liquid Quantity: 50 µg Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Chromatin Immunoprecipitation Sequencing (ChIP-seq), Dot blot (Dot), ELISA (ELISA), Immunofluorescence (IF), peptide array (PA), Western blot (WB) Recommended dilution: 0,5-5 µg/IP (ChIP-seq), 1 : 20 000 (Dot), 1 : 4000 (ELISA), 1 : 500 (IF), 1 : 10 000 (PA), 1 : 1000 (WB) Expected | apparent MW: 15 kDa
- Reactivity
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Confirmed reactivity: Human Predicted reactivity: Arabidopsis thaliana, Mouse, Oryza sativa Not reactive in: No confirmed exceptions from predicted reactivity are currently known - Application Examples
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application example 
Western blot was performed on whole cell (25 μg, lane 1) and histone extracts (15 μg, lane 2) from HeLa cells, and on 1 μg of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively). Anti-H3K36me3 | Histone H3 trimethylated lysine 36 (ChIP grade) antibodies were diluted 1:1 000 in TBS-Tween containing 5% skimmed milk. - Additional Information
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Additional information: Antibody is provided in PBS containing 0,05% azide and 0,05% ProClin 300 Additional information (application): Antibody solution contains PBS with 0,05% sodium azide and 0,05% ProClin 300 - Background
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Background: Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Trimethylation of histone H3K36 is associated with active genes. - Reviews:
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