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AS07 268 | clonality: polyclonal  |  host: rabbit  |  reactivity: higher plants


20 st
Item No:
AS07 268

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product information

This antibody specifically cross-reacts against fucose residues bound to the protein N-glycans in alpha 1,3. This residue is characterisitc of the plant protein N-glycans and is absent in protein N-glycans from animals. This residue is added in the Golgi apparatus.


core fucose residues bound to the N-glycan in alpha 1,3

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum
Format Lyophilized in PBS pH 7.4
Quantity 50 ĩg
Reconstitution For reconstitution add 50 ĩl of sterile water.

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB), immunolocalization (IL)
Related products

AS07 267 | anti-xylose rabbit antibody

Plant and algal protein extraction buffer

Secondary antibodies

Additional information

Alpha (1,3) fucose is present not only in plants but also in some invertebrates (such as nematodes, bees, etc.) . However, cross-reaction with glycoproteins from these organisms is weaker than the one observed in plants. This sugar residue does not exist in mammals, in their endogenous glycoproteins.

application information
Recommended dilution

1 ug/10 ml of incubation buffer, with standard ECL (WB), 1: 40 (IL)

Expected | apparent MW

10 - 100 for various glycoproteins

Confirmed reactivity

higher plants

Predicted reactivity

higher plants

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information


PLA2 (phospholipase 2 from bee venom) which contains only α1.3 fucose, Sigma, product number P9279

Type II - horseradish peroxidase which contains β1.2 Xylose and α1.3 fucose, Sigma, product number P8250

The antibody does not recognize alpha 1,6-fucose.

Selected references Ebert et al. (2015). Identification and Characterization of a Golgi-Localized UDP-Xylose Transporter Family from Arabidopsis. Plant Cell. 2015 Mar 24. pii: tpc.114.133827.
Lehtimäki et al. (2014). Posttranslational modifications of FERREDOXIN-NADP+ OXIDOREDUCTASE in Arabidopsis chloroplasts. Plant Physiol. 2014 Dec;166(4):1764-76. doi: 10.1104/pp.114.249094

application example 1

western blot detection using anti-fucose antibodies

Total cell extract from Arabidopsis thaliana wild type (1) and cell extracts from different mutants defective in fucosyltransferases (2-5) (data not published yet).

Primary antibody has been used at 10 µg/10 ml of incubation buffer. Detection has been done using enchanced chemiluminescence (ECL).

application example 2

Dot blot reaction of anti-Fucose and anti-Xylose antibodies with various controls: Avidin (Fuc+/Xyl+), Fetuin (Fuc-/Xyl-), PLA2 (Fuc+/Xyl-) and Mur1-2 (Fuc-/Xyl+). 2 µl of each extract were spotted on a nitrocellulose membrane, placed on top of 2 WHATMAN filters (one soaked in TBS-T) and dried for 1.5 h at RT. The mem-brane was blocked for 30 min with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and incubated with anti-Fucose(1) (AS07 268, 1:1000) or anti-Xylose(2) (AS07 267, 1:1000) for 30 min and then with secondary anti-rabbit(1:1000) antibody (ALP conjugated, recommended secondary antibody AS09 607). Membrane was washed with TBS-T 3 x 10 minutes before reaction development using alkaline phosphatase reagent BCIP®/NTB premixed solution (Sigma, Prod. No. B6404).

Please follow this link for a more detailed Dot-Blot protocol

application example 3

immunolocalization using anti-fucose antibodies

Immunolocalization using anti-fucose antibodies on a 40µm vibratome section of a 21 day old Arabidopsis thaliana hypocotyl (wood). Fixation: 4% formaldehyde fixation in 1X PME (100 mM PIPES, 1 mM MgSO4, 2 mM EGTA); blocking: 5 % BSA; primary antibody dilution: 1:40; secondary antibody: 1:300 AlexaFluor568 from Invitrogen (Life Technologies), Alexa Fluor® 568 Goat Anti-Rat IgG (H+L); Left panel, immunolabel channel (cyan) overlaid on grayscale channel (0.001% Calcofluor white which stains cellulose and hemicellulose). Right panel, only immunolabelling

Courtesy of Dr. Hardy Hall and Dr. Urs Fischer, Umeå Plant Science Centre, Sweden

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