SUS1 | Sucrose synthase 1

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AS15 2830  |  clonality: polyclonal  |  host: rabbit  |  reactivity: A. thaliana, H. vulgare, Zea mays


15 st
Item No:
AS15 2830

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product information
Background SUS1 (Sucrose synthase 1) is a sucrose-cleaving enzyme that provides UDP-glucose and fructose for various metabolic pathways. Alternative names: ASUS1, ATSUS1. SUS1.

His-tagged, full length Arabidopsis thaliana SUS1, UniProt: P49040, TAIR:AT5G20830

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 ĩl
Reconstitution For reconstitution add 50 ĩl of sterile water.

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

AS13 2748 | anti-SUS4 | Sucrose synthase 4, rabbit antibody
collection of antibodies to carbohydrate metabolism

Plant protein extraction buffer

Secondary antibodies

Additional information Antibody is recognizing recombinant SUS1 protein.
application information
Recommended dilution

1: 10 000 ECL (WB)

Expected | apparent MW

93 kDa

Confirmed reactivity

Arabidopsis thaliana, Hordeum vulgare, Zea mays

Predicted reactivity

Brassica sp., Glycine max, Gossypium sp., Hevea brasiliensis, Jatropha curas, Mangifera indica,  Manihot esculenta, Theobroma cacao, Pisum sativum, populus tomentosa, Ricinus communis

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information
Selected references Kleczkowski LA & Decker DD (2015) Sugar activation for production of nucleotide sugars as substrates for glycosyltransferases in plants. J. Appl. Glycosci. (in press).

application example

western blot using anti-SUS1 antibodies

10 µg of total protein from Arabidopsis thaliana leaf (1) , Hordeum vulgare leaf (2)Zea mays leaf (3) were extracted with Protein Extraction Buffer PEB (AS08 300). Samples were diluted with 1X sample buffer (NuPAGE LDS sample buffer (Invitrogen) supplemented with 50 mM DTT and heat at 70°C for 5 min and keept on ice before loading. Protein samples were separated on 4-12% Bolt Plus gels,  LDS-PAGE and blotted for 70 minutes to PVDF using tank transfer. Blots were blocked immediately following transfer in 2% blocking reagent (GE RPN 2125; Healthcare) or 5% non-fat milk dissolved in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 10 000 (in blocking reagent) for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, and then washed 1x15 min and 3x5 min with TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, recommended secondary antibody AS09 602, Agrisera) diluted to 1:25 000 in blocking reagent for 1h at room temperature with agitation. The blots were washed as above. The blot was developed for 5 min with detection reagent according the manufacturers instructions. Images of the blots were obtained using a CCD imager (VersaDoc MP 4000) and Quantity One software (Bio-Rad). Exposure time was 1 minute.

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