CesA7 (IRX3) | Cellulose synthase A catalytic subunit 7 [UDP-forming]

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AS12 2581 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana, Solanum lycopersicum


21 st
Item No:
AS12 2581

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product information

CesA7 (IRX3) is a catalytic subunit of xylem-specific cellulose synthase, involved in secondary cell wall biosynthesis - cellulose synthase trerminal complexes. It interacts with CESA4 and CESA8 which is required for a functyional complex and localization in secondary cell wall deposition sites. Occurs in secondary cell wall developing tissues such as xylems and interfascicular regions in young plants stems and flowers. Not expressed in leaves, roots and shoots. Synonymes: Protein IRREGULAR XYLEM 3, IRX3, Protein FRAGILE FIBER 5.

Immunogen recombinant Arabidopsis thaliana IRX3 fragment,UniProt: Q9SWW6, TAIR: At5g17420
Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 ĩl
Reconstitution For reconstitution add 50 ĩl of sterile water.

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

AS12 2582 | CesA4 (IRX5) | Cellulose synthase A catalytic subunit 4 [UDP-forming], rabbit antibody

AS12 2580 | CesA8 (IRX1) | Cellulose synthase A catalytic subunit 8 [UDP-forming], rabbit antibody

Plant protein extraction buffer

Secondary antibodies

Additional information This antibody is detecting both, recombinant and edogenous CesA7 (IRX3) protein.
application information
Recommended dilution

1 : 1000 with standard ECL (WB)

Expected | apparent MW

115.7 kDa

Confirmed reactivity

Arabidopsis thaliana, Solanum lycopersicum

Predicted reactivity Brassica napus, Eucalyptus grandis, Nicotiana tabacum, Populus sp.
Not reactive in

no confirmed exceptions from predicted reactivity are currently known

Additional information

to be added when available

Selected references Tsuchiya et al. (2015). Distribution of XTH, expansin, and secondary-wall-related CesA in floral and fruit abscission zones during fruit development in tomato (Solanum lycopersicum). Front Plant Sci. 2015 May 15;6:323. doi: 10.3389/fpls.2015.00323.

application example

western blot using anti-CesA7 (IRX3) antibodies500 mg of Col-0 WT Arabidopsis thaliana stem powder extracted by boiling in 2 mL of 3% SDS loading buffer + 100 mM DTT at 95C for 10 min. Extract was spun at max speed to remove debris and supernatant was taken as crude extract. 25 µL of this was loaded on a 4-15% gel run for 50 min, 150v. . Blots were blocked with 5 % milk  for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 500 over night at 4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, AS09 602 from Agrisera) diluted to 1:5000 in  for 2h at RT with agitation. The blot was washed as above and developed for 5 min with Pierce SuperSignal West Pico kit  according to the manufacturer's instructions. Exposure time was 10 seconds.

Courtesy of Dr. Manoj Kumar, University of Manchester, UK

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