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CesA8 (IRX1) | Cellulose synthase A catalytic subunit 8 [UDP-forming]

265 €
Buy 2 items of this product for 198 €/each
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AS12 2580 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana, Solanum lycopersicum

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AS12 2580

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product information
Background

CesA8 (IRX1) is a catalytic subunit of an enzyme involved in secondary cell wall biosynthesis - cellulose synthase trerminal complexes. It interacts with CESA4 and CESA7 which is required for a functyional complex. The protein is needed for xylem cell wall thickening. Occurs in secondary cell wall developing tissues such as xylems and interfascicular regions. Not expressed in leaves and not found in embryos. Synonymes: Protein IRREGULAR XYLEM 1, IRX1, Protein LEAF WILTING 2, LEW2 .

Immunogen

recombinant Arabidopsis thaliana IRX1 fragment,UniProt: Q8LPK5,TAIR: At4g18780

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 50 ĩl
Reconstitution For reconstitution add 50 ĩl of sterile water.
Storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB), immunofluorescence (IF)
Related products

AS12 2582 | CesA4 (IRX5) | Cellulose synthase A catalytic subunit 4 [UDP-forming], rabbit antibody

AS12 2581 | CesA7 (IRX3) | Cellulose synthase A catalytic subunit 7 [UDP-forming], rabbit antibody

Plant protein extraction buffer

Secondary antibodies

Additional information This antibody is detecting both, recombinant and edogenous CesA8 (IRX1) protein.
application information
Recommended dilution

1 : 1000 with standard ECL (WB)

Expected | apparent MW

111.5 kDa

Confirmed reactivity

Arabidopsis thaliana, Cannabis sativa, Solanum lycopersicum

Predicted reactivity Brassica napus, Populus sp.
Not reactive in

no confirmed exceptions from predicted reactivity are currently known

Additional information
Selected references Zhang et al. (2016). Golgi-localized STELLO proteins regulate the assembly and trafficking of cellulose synthase complexes in Arabidopsis. Nat Commun. 2016 Jun 9;7:11656. doi: 10.1038/ncomms11656.

Tsuchiya et al. (2015). Distribution of XTH, expansin, and secondary-wall-related CesA in floral and fruit abscission zones during fruit development in tomato (Solanum lycopersicum). Front Plant Sci. 2015 May 15;6:323. doi: 10.3389/fpls.2015.00323.


application example

 western blot using anti-CesA(IRX1) antibodies500 mg of Col-0 WT Arabidopsis thaliana stem powder extracted by boiling in 2 mL of 3% SDS loading buffer + 100 mM DTT at 95C for 10 min. Extract was spun at max speed to remove debris and supernatant was taken as crude extract. 25 µL of this was loaded on a 4-15% gel run for 50 min, 150v. . Blots were blocked with 5 % milk  for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 500 over night at 4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, AS09 602 from Agrisera) diluted to 1:5000 in  for 2h at RT with agitation. The blot was washed as above and developed for 5 min with Pierce SuperSignal West Pico kit  according to the manufacturer's instructions. Exposure time was10 seconds.

Courtesy of Dr. Manoj Kumar, University of Manchester, UK


 


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