Tubulin alpha chain
AS10 680 | clonality: polyclonal | host: rabbit | reactivity:Arabidopsis thaliana, Horderum vulgare, Zea mays
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1: 1000 with standard ECL (WB), 1: 500 (IF)
|Expected | apparent MW||
49 | 52 kDa (Arabidopsis thaliana)
Arabidopsis thaliana, Hordeum vulgare, Zea mays
dicots including: Brassica napus, Glycine max, Pisum sativum, Solanum tuberosum, Sorghum bicolor, Ricinus communis, Vitis vinifera, monocots including: Triticum aestivum, Oryza sativa, trees: Picea sitchensis, Populus trichocarpa, moss: Physcomitrella patens, algae: Chlorella vulgaris, Euglena gracilis, Micromonoas pusilla, Ostreococcus lucimarinus
|Not reactive in||
no confirmed exceptions from predicted reactivity known in the moment
Peptide used to elict this antibody is not present in tubulin beta.
Juszczak et al. (2012). Natural genetic variation in the expression regulation of the chloroplast antioxidant system among Arabidopsis thaliana accessions. Physiol. Plant.
5 µg of total protein from Arabidopsis thaliana (1), Hordeum vulgare (2), Zea mays (3) extracted with Agrisera PEB extraction buffer were separated on 4-12 % SDS-PAGE and blotted 1h to PVDF. Blots were blocked with for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 10 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602 ) diluted to 1:25 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL Advance according to the manufacturers instructions (GE Health care). Exposure time was 120 seconds.
Total protein from either rice embryos or rice protoplasts extracted with buffer containing 60mM Tris-HCl (pH8.0), 2% SDS(w/v), 15% Sucrose (w/v) and protease inhibitor 1X were separated on 12 % SDS-PAGE and blotted 1h to PVDF. Blots were blocked with skimmed milk containing TBS 1X for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 overnight at 4 degree with shaking about 40 rpm. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:20 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturers instructions. Exposure time was 10 seconds.
Courtesy of Ho Viet The, PhD Student, Scuola Superiore Sant'Anna (Pisa, Italy).
Tubulin alpha localization in roots of Arabidopsis thaliana. Tubulin alpha (red), nucleus (DAPI white). Plant material has been fixed in para-formaldehyde for 30 minutes. Tissue cleaning has been performed before immunolocalization. Primary antibodies: Agrisera anti-tubulin alpha 1: 500. Secondary antibody: goat anti-rabbit IgG Alexa conjugated (red color), dilution 1: 500. Scale bar – 10 µm.
Courtesy Dr. Taras Pasternak, Freiburg University, Germany
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