Tubulin beta chain

371 €

AS10 681 | clonality: polyclonal | host: rabbit | reactivity:Arabidopsis thaliana, Medicago sativa


6 st
Item No:
AS10 681

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product information

Tubulin beta (TUB) together with alpha tubulin is making up microtubules.


KLH-conjugated peptide derived from available tubulin beta chain sequences (located at a surface loop) including all Arabidopsis thaliana tubulin beta-2/beta-3 chain P29512, beta-4 chain P24636(At5g44340), beta-5 chain P29513(At1g20010), beta-6 chain P29514(At5g12250), beta-7 chain P29515(At2g29550), beta-8 chain P29516(At5g23860), beta-9 chain P29517(At4g20890).

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum
Format Lyophilized in PBS pH 7.4
Quantity 100 ĩg
Reconstitution For reconstitution add 100 ĩl of sterile water

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications immunofluorescence (IF), western blot (WB)
Related products

AS10 680 | tubulin alpha chain

Plant and algal protein extraction buffer

Secondary antibodies

Additional information

Please, note that tubulin beta is only detected in actively dividing meristematic cells (see immunolocalization image below). Therefore to allow detection on a western blot, analyzed material must contain enough meristematic cells with dividing activity.

Signal in a western blot application in Chlamydomonas reinhardtii is obtained with a load of 100 µg/well and a dilution of 1: 500.

application information
Recommended dilution

1: 1000 (IF), 1:500 (WB)

Expected | apparent MW

49 | 49 kDa (Arabidopsis thaliana)

Confirmed reactivity

Arabidopsis thaliana (including suspension cells), Chlamydomonas reinhardii, Medicago sativa

Predicted reactivity

dicots including: Brassica napus, Glycine max, Pisum sativum, Solanum tuberosum, Sorghum bicolor, Ricinus communis, Vitis vinifera, monocots including: Hordeum vulgare, Oryza sativa, Zea mays, trees: Picea sitchensis, Populus trichocarpa, moss: Physcomitrella patens, microalgae:Micromonoas pusilla, Ostreococcus lucimarinus, S. cerevisiae

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

to be added when available

Selected references Heinnickel et al. (2016). Tetratricopeptide repeat protein protects photosystem I from oxidative disruption during assembly. Proc Natl Acad Sci U S A. 2016 Mar 8;113(10):2774-9. doi: 10.1073/pnas.1524040113

application example

plant tubulin beta immunolocalization

Tubulin beta localization in Arabidopsis thaliana epidermis cells. Tubulin beta localized to division plates visualized in green, nucleus with DAPI in blue. Plant material has been fixed in para-formaldehyde for 30 minutes. Tissue cleaning has been performed before immunolocalization. Primary antibodies: Agrisera anti-tubulin beta priamry antibody in dilution 1: 1000 and Agrisera goat anti-rabbit IgG DyLight® 488, secondary antibody in dilution 1: 2000. Scale bar – 10 µm.

detection of plant tubulin in Arabidopsis suspenstion cells

(A) The 3 days old suspension cells of Arabidopsis thaliana have been fixed for 25 minutes with 2% FA, cell wall was partaillly digested with 0.2% Dricelase in MES buffer (pH 5.1) for 30 min at 37°C and the cells were dried on coated slide to omit PM extraction. Following by incubation for 45 minutes with Agrisera anti-tubulin beta chain primary antibody in a dilution of 1: 1000, washing once and 45 min incubation with a secondary goat anti-rabbit Alexa 555 antibody, in a dilution of 1:1000 dilution (Invitrogen). Cells were counterstained witn DAPI and visialized under epi/fluiresecnet microscope (B).

Courtesy Dr. Taras Pasternak, Freiburg University, Germany

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