FT/TSF | Flowering locus T and twin sister of FT

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AS06 198 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana


36 st
Item No:
AS06 198

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product information

In angioperms the 20 kDa protein FT (coded by the gene flowering locus T) promotes flowering under long-day conditions. It has been shown to contribute to flowering initiation upon its expression in leafs followed by transport to apical meristems.


KLH-conjugated synthetic peptide derived fromA.thaliana FT protein sequence(Q9SXZ2, At1g65480); please note that this antibody will cross-react with the highly homologous TSF (twin sister of FT) protein

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum
Format Lyophilized
Quantity 50 ĩg
Reconstitution For reconstitution add 50 ĩl of sterile water.

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
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Additional information

to be added when available

application information
Recommended dilution

1 : 1000, detected with standard ECL (WB)

Expected | apparent MW

20 | 20 kDa for Arabidopsis thaliana

Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity

Betula luminifera, Brassica napa, Hordeum vulgare, Oryza sativa, Populus tomentosa, Solanum tuberosum, Zea mays

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

note that detection for this product is limited by target threshold level

Selected references

to be added when available

application example

35 µg of total leaf protein extracted with PEB (AS08 300) from wt Arabidopsis thaliana (1) and Arabidopsis thaliana transformed with 35S::FT (2) were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 80 min (30V) to PVDF. Filter was blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-FT/TFT (AS06 198, 1:1000, 1h) and secondary anti-rabbit (1:20000, 1h) antibody (HRP conjugated, Abcam) in TBS-T containing 2% low fat milk powder. Antibody incubations were followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signal was detected with standard ECL (GE Healthcare) using a Fuji LAS-3000 CCD (300s, high sensitivity). Please note that this antibody will not detect FT at 35 µg protein loading in the wt leaf material tested.

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