GPA1 | Guanine nucleotide-binding protein subunit alpha 1

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AS12 2370 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana


36 st
Item No:
AS12 2370

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product information
Background GPA1 (G PROTEIN ALPHA-1) is involved as a modulator or transducer in various transmembrane signaling systems. Together with GCR1, may regulate the cell cycle via a signaling cascade  Promotes abscisic acid (ABA) responses in guard cells. But, together with GCR1 and GB1, acts as a negative regulator of ABA during seed germination and early seedling development. Involved in the blue light (BL) signaling. Alternative name: GP-alpha-1.

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana GPA1, UniProt: P18064, TAIR: AT2G26300

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum
Format Lyophilized in PBS pH 7.4
Quantity 50 µg

For reconstitution add 50 ĩl of sterile water.


Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western Blot (WB)
Related products

Antibodies to ABA and proteins involved in ABA metabolism

Antibodies to proteins involved in photomorphogenesis

Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution

1 : 4000 with ECL (WB)

Expected | apparent MW

49.3 kDa

Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity Brassica napus, Capsicum annuum , Cynara cardunculus var. scolymus, Eschscholzia californica, Glycine max, Gossypium hirsutum, Hordeum vulgare, Lupinus luteus, Medicago truncatula, Morus notabilis, Nicotiana benthamiana, Nicotiana tabacum, Oryza sativa, Pisum sativum, Phaseolus vulgaris, Populus trichocarpa, Ricinus communis , Solanum lycopersicum, Solanum tuberosum, Sorghum bicolor, Spinacia oleracea, Theobroma cacao, Triticum aestivum, Zea mays, Zostera marina, Vitis vinifera
Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Additional information

The nature of a 37 kDa cross-reacting band is not known. 

Selected references

To be added when available, antibody released in April 2016. 

Application example

western blot using anti-GPA1 polyclonal antibodies
25 µg of total protein from the indicated Arabidopsis thaliana wilde type and gpa1-3 mutant were extracted with the extraction buffer (250 mM sucrose, 100mM HEPES-KOH pH 7.5, 5% glycerol, 1mM Na2MoO4 x 2H2O, 25mM NaF, 10mM EDTA, 1mM DTT, 0.5%Triton X-100 ,  protease inhibitor cocktail) and denatured with SDS loading dye (50mM Tris-HCl pH6.8, 100 mM DTT, 2%SDS, 10% glycerol, 0.025% bromophenol blue) at 70°C for 2-5 min were separated on  10% SDS-PAGE  and blotted 1h to PVDF using tank transfer. Blots were blocked with Tris-buffered saline containing 0.05% Tween-20 (TBS-T)  and 10% skimmed milk powder for 2h and 20 min. at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 5 000 overnight at 4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 5 times for 15 min in TBS-T with milk powder at RT with agitation. Blot was incubated in The secondary antibody (Agrisera Goat anti-rabbit IgG (H&L) HRP conjugate, AS09 602, 0.91 µg/µl) was diluted 1:5000 in the same solution and incubated on the membrane at room temperature for 2h. The membrane was then washed 5 times 15min with TBS-T (no milk powder) and the blot was developed using Super Signal West Pico and Femto (mixed 1:1) Chemiluminescent Substrate (Thermo Scientific). Exposure time was:  10 minutes.

Courtesy of Dr. Elena Petusching, Georg-August-University Goettingen, Germany

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