CrPDAT1 | Phospholipid: diacylglycerol acyltransferase

345 €

AS12 1875  | clonality: polyclonal  |  host: rabbit  |  reactivity: Chlamydomonas reinhardtii


17 st
Item No:
AS12 1875

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product information
Background PDAT1 is an enzyme phospholipid: diacylglycerol acyltransferase, which catalyzes TAG (triglycerols) synthesis. It has a strong lipase activity with a broad substrate specificity.
Immunogen recombinant CrPDAT1 without transmembrane domains, overexpressed in E.coli,
Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 350 ĩl
Reconstitution For reconstitution add 350 ĩl of sterile water.

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

antibody collection to other Chlamydomonas proteins

Algal protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution

1 : 250 with standard ECL (WB) and load per well of up to 30 ug

Expected | apparent MW

140 kDa

Confirmed reactivity

Chlamydomonas reinhardtii

Predicted reactivity Chlamydomonas reinhardtii
Not reactive in

no confirmed exceptions from predicted reactivity are currently known

Additional information

to be added when available

Selected references

Yoon et al (2012). Phospholipid:Diacylglycerol Acyltransferase Is a Multifunctional Enzyme Involved in Membrane Lipid Turnover and Degradation While Synthesizing Triacylglycerol in the Unicellular Green Microalga Chlamydomonas reinhardtii.  Plant Cell, Oct 2012.

application example

western blot using anti-PADT antibody

Total proteins (containing 2.5 to 30 ug ) from Chlamydomonas cells extracted with lysis buffer (50 mM Tris-HCl, pH 6.8, containing 2% SDS and 10 mM EDTA and a protease inhibitor cocktail) were separated on 10 % SDS-PAGE and transferred onto a nitrocellose blot over night at 4°C. Blots were blocked with blocking buffer ( 5% (w/v) non-fat dry milk powder in TBS-T) for 2 hrs at room temperature (RT) with agitation. Blot was incubated in the primary antibody (∆TMCrPDAT) at a dilution of 1:250 over night at 4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then whashed 5 times for 15 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Bio-Rad) diluted to 1:5000 in the same buffer for 1h at RT with agitation. The blot was washed as above and developed for 5 min with Chemiluminescence detection kit (Bio-Rad) according to the manufacturers instructions. An imaging system (ChemiDoc XRS; Bio-Rad) was used to quantitatively and qualitatively analyze protein blot. Exposure time was 30 seconds.

Courtesy of Dr. Kangsup Yoon, Arizona State University.

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