LOX | Lipoxygenase (Affinity purified)

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AS06 128A  |  clonality: polyclonal  |  host: rabbit  |  reactivity: A.thaliana, G. max


11 st
Item No:
AS06 128A

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product information

Lipoxygenases (LOXs; EC, synonym: lipoxydases) are a family of enzymes that catalyze oxygenation of polyunsaturated fatty acids (PUFAs) into lipidhydroperoxides (LOOHs) involved in responses to stresses. LOXs has been found to play a role in plant growth and development, senescence as well as can be activated in response to environamental stress (drought, heavy metals).Synonymes: linoleate, oxygen oxidoreductase.


native lipoxygenase, type I-B, purified from Glycine max (Sigma, product number L7395) P08170

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum
Format Lyophilized PBS pH 7.4
Quantity 50 ĩg
Reconstitution For reconstitution of lyophilized unit please add 50 ĩl of sterile water.

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

AS06 128 | anti-LOX | lipoxygenase, rabbit antibodies (not purified serum)

AS07 258 | anti-LOX-C | chloroplastic lipoxygenase

AS08 340 | anti-LOX2 | lipoxygenase 2 protein

Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution

1:5000 (WB)

Expected | apparent MW

54 (subunit), 108 (native enzyme)

Confirmed reactivity

Arabidopsis thaliana, Glycine max

Predicted reactivity

Lathyrus undulatus, Malus x domestica, Solanum tuberosum

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information
Selected references to be added when available, antibody released in December 2014

application example

western blot using anti-LOX antibodies

10 µg of total protein from Arabidopsis thaliana (1), native LOX protein from Glycine max (2,3) total protein from all the samples were extracted with Protein Extraction Buffer PEB (AS08 300). Samples were diluted with 1X sample buffer (NuPAGE LDS sample buffer (Invitrogen) supplemented with 50 mM DTT and heat at 70 C for 5 min and keep on ice before loading. Protein samples were separated on NuPAGE 4-12% Tris-Bis gel (Invitrogen) LDS-PAGE and blotted for 1h to 1.5h on PVDF using tank transfer. Blots were blocked immediately following transfer in 2% blocking reagent (GE RPN 2125; Healthcare) or 5% non-fat milk dissolved in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 5 000 (in blocking reagent) for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, and then washed 1x15 min and 3x5 min with TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, recommended secondary antibody AS09 602) diluted to 1:25 000 in blocking reagent for 1h at room temperature with agitation. The blots were washed as above. The blot was developed for 5 min with ECL detection reagent according the manufacturers instructions. Images of the blots were obtained using a CCD imager (FluorSMax, Bio-Rad) and Quantity One software (Bio-Rad).

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