AS10 674 | clonality: polyclonal | host: rabbit | reactivity:Arabidopsis thaliana
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|Expected | apparent MW||
28 | 30 kDa
Cucumis sativus, Hordeum vulgare, Musa sp., Oryza sativa, Phaseolus vulgaris, Physcomitrella patens, Solanum tuberosum, Ricinus communis,Triticum aestivum, Zea mays
|Not reactive in||
Lemna minor, Ostreococcus tauri
antibody is recognizing a chloroplastic form of plant ferritin
10 µg of total protein from Arabidopsis thaliala whole seedlings, various cellular fractions, extracted with (see below*) were separated on 15 % SDS-PAGE and blotted 1h to PVDF. Blots were blocked with 5% Non fat dairy milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 3000 overnight at 4°C with agitation. The antibody solution was decanted and the blot was washed four times for 5 min inPBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:15 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturers instructions. Exposure time was 180 seconds.
*Following is the protein extraction buffer composition that was used to extract the total protein from the whole seedlings:50mM Tris, 4% SDS, 1% PVPP , 5% Glycerol, 1mM PMSF, 2mM Pefabloc.
Courtesy of Anshika Jain and Erin Connolly, University of South Carolina, USA
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