Phly | DNA photolyase (At4g25290)

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AS15 2864   | clonality: polyclonal  |  host: rabbit  |  reactivity: Arabidopsis thaliana


51 st
Item No:
AS15 2864

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product information
Background Protein belongs to DNA photolyases and functions in DNA repair.
Immunogen KLH-conjugated peptide derived from Arabidopsis thaliana DNA photolyase, UniProt: F4JSJ6, TAIR: AT4G25290, located towards C-terminal part of this protein
Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum
Format Lyophilized in PBS pH 7.4
Quantity 50 ĩg
Reconstitution For reconstitution add 50 ĩl of sterile water.

Lyophilized antibody can be stored at -20°C for up to 3 years. Re-constituted antibody can be stored at 4°C for several days to weeks.
Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

AS15 2863 | anti-Phly | DNA photolyase (At4g25290), rabbit antibodies (peptide used to elicit this antibody is located in N-terminal part of the protein)
collection of antibodies to DNA/RNA cell cycle

Plant and algal protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution

1 : 1000 with ECL (WB)

Expected | apparent MW

78 | 90 kDa

Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity 6xHis
Not reactive in

no confirmed exceptions from predicted reactivity are currently known

Additional information
Selected references

to be added when available antibody available in March 2016

application example

western blot using anti-Phly (At4g25290) antibodies

2,5 µg of total protein from Arabidopsis thaliana wilde type darkness (1), wilde type light (2) and insertion mutants:  SALK_056328C darkness (3), SALK_056328C light (4), extracted with 0.1 M Tris-HCl pH 8.5, 4% SDS, 2% (v/v) 2-mercaptoethanol, 2 mM phenylmethylsulfonyl fluoride and denatured with Laemmli buffer at 95oC for 10 min were separated on 12% SDS-PAGE and blotted 2h to PVDF using semi-dry transfer. Blots were blocked with 5% milk PBS-T (Tween 0.5%) for 30 min. at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1:1000 overnight at 4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 3 times for 10 min in 5% milk PBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera) diluted to 1:25 000 in 5 %milk PBS-T for 1h at RT with agitation. The blot was rinsed briefly twice, then washed 3 times for 10 min in PBS-T at RT with agitation. Blot was developed for 5 min with Clarity™ Western ECL Blotting S ubstrate (Biorad). Exposure time was 5 minutes.

Courtesy of Dr. Justyna Łabuz, Department of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Poland

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