RPS2 | Ribosomal protein S2 (mitochondrial)

320 €
AS16 3832 | clonality: polyclonal | host: rabbit | ractivity: Oryza sativa


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Item No:
AS16 3832

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product information
Background RPS2 (Ribosomal protein S2) is encoded by mitochondrial genome and involved in translation process. Alternative name: Ribosomal protein small subunit 2.
Immunogen KLH-conjugated synthetic peptide chosen from Oryza sativa RPS2 protein sequence UniProt: Q2F981; LOC_Osm1g00310.1
Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 100 µl
Reconstitution For reconstitution add 100 µl of sterile water.
Storage Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles.
Tested applications Western Blot (WB)
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antibodies to proteins involved in translation
Additional information
application information
Recommended dilution 1: 500 (WB)
Expected | apparent MW

54 kDa

Confirmed reactivity

Oryza sativa

Predicted reactivity Oryza sativa
Not reactive in
Additional information
Selected references To be added when available, antibody released in April 2016.

Application example

western blot using anti-RPS2 (mitochondrial) antibdodies

50-100 µg of total protein from Oryza sativa flag leaf, roots or fringe extracted with protein extraction reagent were separated on 15% SDS-PAGE and blotted 1h to PVDF using wet transfer. Blots were blocked with 1×TBST+5% Nonfat dry milk for 1h at 37°C with agitation. Blot was incubated in the primary antibody at a dilution of 1: 500 in 1×TBST+5% Nonfat dry milk for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min. and 3 times for 5 min. in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG HRP/conjugated) diluted to 1:15 000 in 1x TBST + 5% Nonfat dry milk for 2h at RT with agitation. The blot was washed as above and developed for 5 min. with ECL according to the manufacturer's instructions. Exposure time was 10 seconds.

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