H3K18me1 | Histone H3 (monomethylated Lys18)

440 €
AS16 3181 | clonality: polyclonal | host: rabbit | reactivity: chicken, C.elegans, D. melanogaster, human, mouse, plant, rat, Xenopus sp.


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Item No:
AS16 3181

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product information
Background Chromatin is the arrangement of DNA and proteins in which chromosomes are formed. Correspondingly, chromatin is formed from nucleosomes, which are comprised of a set of four histone proteins (H2A, H2B, H3, H4) wrapped with DNA. Chromatin is a very dynamic structure in which numerous post-translational modifications work together to activate or repress the availability of DNA to be copied, transcribed, or repaired. These marks decide which DNA will be open and commonly active (euchromatin) or tightly wound to prevent access and activation (heterochromatin). Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine.
Immunogen KLH-conjugated synthetic peptide
Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum
Quantity 50 ĩg
Storage Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Tested applications chromatin immunoprecipiation (ChIP), Dot blot (Dot), immunofluorescence (IF), western blot (WB)
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Additional information This antibody preparation is provided in 20 mM Potassium Phosphate pH 7.2, 150 mM NaCl, 0,01% sodium azide and 30% glycerol.
application information
Recommended dilution 2-5 µg/million cells (ChIP), 1: 1000 (Dot), 1: 50 (IF), 1: 100 (IHC), 1: 500 (WB)
Expected | apparent MW

15 kDa

Confirmed reactivity


Predicted reactivity C.elegans, chicken, D. melanogaster, mouse, plant, rat, Xenopus sp.
Not reactive in No confirmed exceptions from predicted reactivity known at the moment
Additional information To be added when available
Selected references To be added when available, antibody released in February 2016.

application example

Dot blot using anti-H3K18me1 | Histone H3 (monomethyl Lys18) polyclonal antibodies
Dot Blot using anti-H3K18me1 antibodies. Lane 1: unmodified. Lane 2: K18Me1. Lane 3: K18Me2. Lane 4: K18Me3. Lane 5: K18ac. Load: 1, 10, and 100 picomoles of peptide. Primary antibody used at a 1:1 000 dilution for 45 min at 4°C. Secondary antibody: Dylight®488 rabbit secondary antibody at 1:10 000 for 45 min at RT.

immunofluorescence using anti-H3K18me1 | Histone H3 (monomethyl Lys18)  polyclonal antibodies

Immunofluorescence using anti-H3K18me1 antibodies. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody used at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10 000 for 45 min at RT. Localization: Histone H3K18me1 is nuclear and chromosomal. Staining: Histone H3K18me1 is expressed in green, nuclei and actin are counterstained with DAPI (blue) and Phalloidin (red).

western blot using anti-H3K18me1 | Histone H3 (monomethyl Lys18)  polyclonal antibodies
Western Blot using anti-H3K18me1 antibodies. 30 μg of NIH-3T3 histone extracts. Primary antibody used at a 1:500 dilution overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10 000 for 45 min at RT.