H3K27ac | Histone H3 acetylated lysine 27 (ChIP grade)

420 €
AS16 3195 | clonality: polyclonal | host: rabbit | reactivity: A.thaliana, human, mouse, rat


0 st
Item No:
AS16 3195

Info: Product suggestions Add review
product information
Background Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Acetylation of histone H3K27 is associated with active promoters and enhancers.
Immunogen KLH-conjugated synthetic peptide
Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS with 0.05 % sodium azide
Quantity 50 ĩg
Storage Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Tested applications chromatin immunoprecipiation (ChIP/ChIP-seq), Dot blot (Dot), ELISA (ELISA), immunofluorescence (IF), peptide array (PA), western blot (WB)
Related products AS16 3190-10 | anti-H3K4me3 | histone H3, trimethylated lysine 4 (H3K4me3) (10 µg), rabbit antibodies

AS16 3187 | anti-H3K56me1 | Histone H3 (monomethyl Lys56), polyclonal antibodies
AS16 3182
| anti-H3K18me2 | Histone H3 (dimethyl Lys18), rabbit antibodies
AS16 3183 | anti-H3K18me3 | Histone H3 (trimethyl Lys18), rabbit antibodies

AS16 3181 | anti-H3K18me1 | Histone H3 (monomethyl Lys18), rabbit antibodies
AS16 3168 | anti-H3T3pK4me1 | Histone H3 (monomethyl Lys4, p Thr3), rabbit antibodies
AS16 3169 | anti-H3T3pK4me2 | Histone H3 (dimethyl Lys4, p Thr3), rabbit antibodies
AS16 3172 | anti-H3K4me3T6p | H3 (trimethyl Lys4, p Thr6), rabbit antibodies

AS16 3197 | anti-anti-H3core, monoclonal antibodies
AS15 2855 | anti-H3 | histone H3, chicken polyclonal antibodies
AS10 710 | anti-H3 | histone H3, rabbit polyclonal antibodies
AS10 710PRE | H3 | histone H3, pre-immune serum to H3 antibody AS10 710

collection of antibodies to epigenetics
Additional information Antibody is provided in PBS containing 0.05% azide and 0.05% ProClin 300.
application information
Recommended dilution 0.5-5 µg/IP (ChIP/ChIP-seq), 1: 20 000 (Dot), 1: 500 (ELISA), 1: 500 (IF),  1: 1000 (WB)
Expected | apparent MW

15 kDa

Confirmed reactivity


Predicted reactivity A.thaliana, mouse, rat
Not reactive in No confirmed exceptions from predicted reactivity known at the moment
Additional information Antibody solution contains PBS with 0.05% sodium azide and 0.05% ProClin 300.
Selected references To be added when available, antibody released in February 2016.

application example

Dot blot using anti-H3K27ac | Histone H3 acetylated lysine 27 (ChIP grade)  polyclonal antibodies

Dot blot was used to to test the cross reactivity of anti-H3K27ac antibodies. Peptides containing other histone modifications and the unmodified H3K27 were used. 100 to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20 000. Results show a high specificity of the antibody for the modification of interest.