H3K4me3 | Histone H3, trimethylated lysine 4 (H3K4me3)

356 €
AS16 3192 | clonality: polyclonal | host: rabbit | reactivity: A.thaliana, human, mouse


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Item No:
AS16 3192

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product information
Background Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called “histone code”. Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Trimethylation of histone H3K4 is associated with active promoters.
Immunogen KLH-conjugated synthetic peptide
Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum
Quantity 50 ĩg
Storage Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Tested applications chromatin immunoprecipiation (ChIP/ChIP-seq), Dot blot (Dot), immunofluorescence (IF), western blot (WB)
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collection of antibodies to epigenetics
Additional information Antibody is provided in PBS containing 0.05% azide and 0.05% ProClin 300.
application information
Recommended dilution 1-5 µg/IP (ChIP/ChIP-seq), 1: 2000 (Dot), 1: 500 (IF), 1: 500 (WB)
Expected | apparent MW

15 kDa

Confirmed reactivity


Predicted reactivity
Not reactive in No confirmed exceptions from predicted reactivity known at the moment
Additional information To be added when available
Selected references To be added when available, antibody released in February 2016.

application example

ChIP using anti-H3K4me3 | histone H3, trimethylated lysine 4 (H3K4me3) polyclonal antibodies
ChIP assays were performed using human HeLa cells, and anti- H3K4me3 antibodies and optimized PCR primer pairs for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit, using sheared chromatin from 1 million cells. A titration consisting of 1, 2, 5 and 10 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for the promoter of the active genes GAPDH and EIF4A2, used as positive controls, and for exon 2 of the inactive myoglobin (MB) gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). These results are in accordance with the observation that trimethylation of K4 at histone H3 is associated with the promoters of active genes.