H3K4me3T6p | H3 (trimethylated Lys4, p Thr6)

440 €
AS16 3172 | clonality: polyclonal | host: rabbit | reactivity: C.elegans, D. melanogaster, human, mouse, plant, rat, Xenopus sp.


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Item No:
AS16 3172

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product information
Background Methylation of lysine 4 on H3 (H3K4Me) and phosphorylation of threonine 3 (H3T3p) are known marks of transcriptional activation and mitosis. H3K4 has many known modifying enzymes (Set1, Set7/9, MLL, ASH1).
Immunogen KLH-conjugated synthetic peptide
Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum
Quantity 50 ĩg
Storage Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Tested applications chromatin immunoprecipiation (ChIP), Dot blot (Dot), immunofluorescence (IF), immunohistochemistry (IHC), western blot (WB)
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Additional information This antibody preparation is provided in 20 mM Potassium Phosphate pH 7.2, 150 mM NaCl, 0,01% sodium azide and 30% glycerol.
application information
Recommended dilution 2-5 µg/million cells (ChIP), 1: 1000 (Dot), 1: 50 (IF), 1: 50 (IHC), 1: 500 (WB)
Expected | apparent MW

15 kDa

Confirmed reactivity

C.elegans, human

Predicted reactivity D. melanogaster, mouse, plant, rat, Xenopus sp.
Not reactive in No confirmed exceptions from predicted reactivity known at the moment
Additional information To be added when available
Selected references To be added when available, antibody released in February 2016.

application example

ChIP using anti-H3K4me3T6p  antibodies

Chromatin Immunoprecipitation
using anti-H3K4me3T6p antibodies. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2 μg of H3K4me3T6p antibody alongside a no antibody (No Ab) control, DNA was measured by qPCR and normalized to total input.
Dot blot using anti-H3K4me3T6p antibodies

Dot Blot using anti-H3K4me3T6p antibodies. Lane 1: Unmodified. Lane 2: T6p. Lane 3: K4Me1T6p. Lane 4: K4Me2T6p. Lane 5: K4Me3T6p. Load: 3, 1, and 0.3 picomoles of peptide. Primary antibody used at a 1:1 000 dilution for 45 min at 4°C. Secondary antibody: Dylight®488 rabbit secondary antibody at 1:10 000 for 45 min at RT.

IF using anti-H3K4me3T6p antibodies

Immunofluorescence using anti-H3K4me3T6p antibodies. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody used at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10 000 for 45 min at RT. Localization: Histone H3K4me3T6p is nuclear and chromosomal. Staining: H3K4me3T6p is expressed in green and the nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight® 594 (red).

western blot using anti-H3K4me3T6p antibnodies
Western Blot using anti-H3K4me3T6p antibodies. 30 μg C. elegans embryo lysate. Primary antibody at 1:500 overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10 000 for 45 min at RT.