H3K9ac | Histone H3, acetylated lysine 9

350 €
AS16 3198 | clonality: polyclonal | host: rabbit | reactivity: human, mouse, P. falciparum, Populus sp. 


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Item No:
AS16 3198

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product information
Background Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases.
Immunogen KLH-conjugated synthetic peptide
Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS with 0.05 % sodium azide
Quantity 50 ĩg
Storage Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Tested applications chromatin immunoprecipiation (ChIP/ChIPseq), Dot blot (Dot), ELISA (ELISA), immunofluorescence (IF), western blot (WB)
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collection of antibodies to epigenetics
Additional information Antibody is provided in PBS containing 0.05% azide and 0.05% ProClin 300.
application information
Recommended dilution 2-5 µg/IP (ChIP), 1: 20 000 (Dot),  1: 1000 (ELISA), 1: 500 (IF), 1: 1000  (WB)
Expected | apparent MW
Confirmed reactivity


Predicted reactivity mouse, P. falciparum, Populus sp. 
Not reactive in No confirmed exceptions from predicted reactivity known at the moment
Additional information To be added when available
Selected references To be added when available, antibody released in February 2016.

application example

western blot using anti-H3K9ac | Histone H3, acetylated lysine 9  polyclonal antibodies
Dot Blot analysis was performed to test the cross reactivity of anti-H3K9ac antibodies with peptides containing other histone modifications and the unmodified H3K9 sequence. 100 to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure shows a high specificity of the antibody for the modification of interest. The antibody recognizes the H3K9 acetylation, both in the presence and the absence of the K14 acetylation