H3K9me2 | Histone H3 dimethylated lysine 9

356 €
AS16 3194 | clonality: polyclonal | host: rabbit | reactivity: A.thaliana, human, mouse, O. sativa , S. lycopersicum


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Item No:
AS16 3194

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product information
Background Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Dimethylation of histone H3K9 is more present in silent genes.
Immunogen KLH-conjugated synthetic peptide
Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS and 0.05% sodium azide
Quantity 50 ĩg
Storage Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Tested applications chromatin immunoprecipiation (ChIP), Dot blot (Dot), ELISA (ELISA), immunofluorescence (IF), western blot (WB)
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collection of antibodies to epigenetics
Additional information Antibody is provided in PBS containing 0.05% azide and 0.05% ProClin 300.
application information
Recommended dilution 1-5 µg/IP (ChIP/ChIP-seq), 1: 20 000 (Dot), 1: 1000 (ELISA), 1: 500 (IF),  1: 1000 (WB)
Expected | apparent MW

15 kDa

Confirmed reactivity


Predicted reactivity A.thaliana, mouse, O. sativa , S. lycopersicum
Not reactive in No confirmed exceptions from predicted reactivity known at the moment
Additional information Antibody solution contains PBS with 0.05% sodium azide and 0.05% ProClin 300.
Selected references To be added when available, antibody released in February 2016.

application example

Dot blot using anti-H3K9me2 | Histone H3 dimethylated lysine 9 polyclonal antibodies

Dot blot
analysis to test the cross reactivity of anti-H3K9me2 antibodies with peptides containing other modifications of histone H3 and the unmodified H3K9 sequence. 100 to 0.2 pmol of peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:20 000.