H3T3pK4me1 | Histone H3 (p Thr3, monomethylated Lys4)

440 €
AS16 3168 clonality: polyclonal  |  host: rabbit  |  reactivity: C.elegans, D. melanogaster, human, mouse, plant, rat, Xenopus sp.


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Item No:
AS16 3168

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product information
Background Methylation of lysine 4 on H3 (H3K4Me) and phosphorylation of threonine 3 (H3T3p) are known marks of transcriptional activation and mitosis. H3K4 has many known modifying enzymes (Set1, Set7/9, MLL, ASH1).
Immunogen KLH-conjugated synthetic peptide
Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum
Quantity 50 ĩg
Storage Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Tested applications Dot blot (dot), immunofluorescence (IF), immunohistochemistry (IHC), western blot (WB)
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Additional information This antibody preparation is provided in 20 mM Potassium Phosphate pH 7.2, 150 mM NaCl, 0,01% sodium azide and 30% glycerol.
application information
Recommended dilution 1: 1000 (Dot), 1: 100 (IF), 1: 100 (IHC), 1: 1000 (WB)
Expected | apparent MW

15 kDa

Confirmed reactivity


Predicted reactivity C.elegans, D. melanogaster, mouse, plant, rat, Xenopus sp.
Not reactive in No confirmed exceptions from predicted reactivity known at the moment
Additional information To be added when available
Selected references To be added when available, antibody released in February 2016.

application example

Dot blot using anti-H3T3pK4me1 polyclonal antibodies
Dot Blot using H3T3pK4me1 antibodies. Load: 1, 10, and 100 picomoles of the different peptides. Primary antibody used at a 1:1 000 dilution for 45 min at 4°C. Secondary antibody: HRP rabbit secondary antibody at 1:40 000 for 45 min at RT.

Immunofluorescence using H3T3pK4me1 polyclonal antibodies

Immunofluorescence using anti-H3T3pK4me1 antibodies. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody: used at a 1:100 dilution for 1 h at RT. Secondary antibody: Dylight 488 secondary antibody at 1:10 000 for 45 min at RT. Localization: Histone H3T3pK4me1 is nuclear and chromosomal. Staining: Histone H3T3pK4me1 is expressed in green while the nuclei and aplpha- tubulin were coexpressed with DAPI (blue) and Dylight 550 (red).

Western blot using anti-H3T3pK4me1 antibodies

Western Blot using anit-H3T3pK4me1 antibodies. Lane 1: HeLa histone extracts. Lane 2. NIH 3T3 histone extracts Lane 3. C. elegans embryo lysate. Load: 30 μg per lane. Primary antibody used at a 1:1 000 dilution overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10 000 for 45 min at RT.