H3T3pK4me2 | Histone H3 (dimethylated Lys4, p Thr3)

440 €
AS16 3169 clonality: polyclonal  |  host: rabbit  |  reactivity: C.elegans, D. melanogaster, human, mouse, plant, rat, Xenopus sp.


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Item No:
AS16 3169

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product information
Background Methylation of lysine 4 on H3 (H3K4Me) and phosphorylation of threonine 3 (H3T3p) are known marks of transcriptional activation and mitosis. H3K4 has many known modifying enzymes (Set1, Set7/9, MLL, ASH1).
Immunogen KLH-conjugated synthetic peptide
Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum
Quantity 50 ĩg
Storage Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Tested applications Dot blot (Dot), chromatine immunoprecipiation (ChIP), immunofluorescence (IF), immunohistochemistry (IHC), western blot (WB)
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Additional information This antibody preparation is provided in 20 mM Potassium Phosphate pH 7.2, 150 mM NaCl, 0,01% sodium azide and 30% glycerol.
application information
Recommended dilution 2-5 µg/milion cells (ChiP), 1: 1000 (Dot), 1: 50 (IF), 1: 50 (IHC), 1: 500 (WB)
Expected | apparent MW

15 kDa

Confirmed reactivity


Predicted reactivity C.elegans, D. melanogaster, mouse, plant, rat, Xenopus sp.
Not reactive in No confirmed exceptions from predicted reactivity known at the moment
Additional information To be added when available
Selected references To be added when available, antibody released in February 2016.

application example

ChIP using H3T3pK4me2 antibodies
Chromatin Immunoprecipitation usingH3T3pK4me2 antibodies. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2 μg of H3T3pK4me2 and 20 μl of magnetic beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and normalized to the input chromatin.

Immunofluorescence using anti-H3T3pK4me2 antibodies

Immunofluorescence using anti-H3T3pK4me2 antibodies. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody was used at a 1:50 dilution for 1 h at RT. Secondary antibody: Dylight® 488 secondary antibody at 1:10 000 for 45 min at RT. Localization: Histone H3T3pK4me2 is nuclear and chromosomal. Staining: H3T3pK4me2 is expressed in green while the nuclei and aplpha-tubulin were coexpressed with DAPI (blue) and Dylight® 550 (red).

western blot using anti-H3T3pK4me2 antibodies
Western Blot using anti-H3T3pK4me2 antibodies. 30 μg of NIH-3T3 histone extracts. Primary antibody used at a 1:500 dilution overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10 000 for 45 min at RT.