AGO10 | Argonaute 10

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AS15 3071 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana


33 st
Item No:
AS15 3071

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product information

AGO10 is involved in miRNA binding, and in RNA-mediated posttranscriptional gene silencing (PTGS).


KLH-conjugated synthetic peptide derived from Arabidopsis thaliana AGO10 protein sequence, Uniprot: Q9XGW1, TAIR: AT5G43810

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS pH 7.4.
Quantity 50 µl

For reconstitution add 50 ĩl of sterile water.


Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
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collection of antibodies to micro RNA

Plant protein extraction buffer

Secondary antibodies

Additional information TCA acetone total protein precipitation method
application information
Recommended dilution

1: 10 000 (WB)

Expected | apparent MW

110.9 kDa

Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity

A. lyrata, B. napus, C. rubella, C. clementina, C. sinensis, E. salsugineum, G. arboreum, G. raimondii

Not reactive in

Zea mays

Additional information

AGO expression may be cell/tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Seedlings can be used as a negative control.

Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure.

Selected references To be added when available, antibody released in April 2016. 

Application example

western blot using anti-AGO10 antibodies

50 µg of total protein from Arabidopsis thaliana inflorescences were extracted with extraction buffer (50 mM Tris pH7.5; 150 mM NaCl; 1 mM EDTA; 10 % v/v Glycerin; 1 mM DTT, 1x Complete Protease Inhibitor Cocktail, Roche) and denatured with Laemmli buffer at 95ºC 5 min. were separated on 10% SDS-PAGE and blotted 1.5 h to PVDF using tank transfer. Blots were blocked with blocking buffer (3% milk powder; 1x TBS; 0.1% Tween-20) 1 h at RT with agitation. Blot was incubated in the primary antibody at a dilution of 1:10 000 ON at 4ºC with agitation. The antibody solution was decanted and the blot was rinsed briefly and then washed tree times for 15 min. in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:20 000 in blocking buffer for 1h at RT with agitation. The blot was washed as above and developed for 5 min with Amersham ECL Prime and expose to Amersham Hyperfilms ECL for 5 minutes.

Courtesy of Dr. Dr. Pablo Manavella, Instituto de Agrobiotecnología del Litoral (IAL), Argentina

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