AGO5 | Argonaute 5

379 €

AS10 671  |  clonality: polyclonal  |  host: rabbit  |  reactivity: Arabidopsis thaliana


12 st
Item No:
AS10 671

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product information

AGO5 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi). Probably involved in antiviral RNA silencing.


peptide of Arabidopsis thaliana AGO5 UniProt: Q9SJK3, TAIR:AT2G27880

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS pH 7.4. 
Format Lyophilized
Quantity 200 ĩg
Reconstitution For reconstitution add 200 ĩl of sterile water.

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB), immunoprecipitation (IP)
Related products

AS09 527 | Anti-AGO1 | argonaute 1, rabbit antibody
AS09 527-ALP | Anti-AGO1 | argonaute 1 (40 µg, ALP-conjugated) 
AS09 527B | Anti-AGO1 | argonaute 1 (40 µg, Biotin conjugated)
AS09 527-HRP | Anti-AGO1 | argonaute 1 (40 µg, HRP-conjugated) 
AS14 2776 | Anti-AGO1 | argonaute 1 (Chlamydomonas), rabbit antibody
AS09 527P | AGO1 | argonaute 1 | Blocking peptide
AS13 3682 | Anti-AGO2 | argonaute 2, rabbit antibody
AS09 617 | Anti-AGO4 argonaute 4, rabbit antibody
AS10 672 | Anti-AGO6 | argonaute 6, rabbit antibody
AS10 673 | Anti-AGO9 | argonaute 9, rabbit antibody
AS15 3071 | Anti-AGO10 | argonaute 10, rabbit antibody

collection of antibodies to micro RNA

Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution

1: 1500 (WB),  5 ug per gram floral tissue (IP)

Expected | apparent MW

111 | 111 kDa

Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity

Arabidopsis thaliana

Not reactive in

Zea mays

Additional information

AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure. 

TCA acetone total protein precipitation method

Selected references To be added when available, antibdy released in August 2012.

application example

western blot using anti-AGO5 antibodies on Arabidopsis thaliana
Arabidopsis thaliana total protein extracted by TCA-acetone precipitation from floral buds of Col-0 (which should be more enriched in AGO5) was saturated in 8M urea were separated on 10% SDS-PAGE and blotted for 1hour to 0.2 µm nitrocellulose at 100V using wet transfer system. Blots were blocked with 0.5% cold fish gelatin for 1hr at room temp with agitation. Blot was incubated in the primary antibody a dilution of 1:250 overnight at 4C with agitation. The blots were washed with 3X 15min TBS-TT at RT with agitation. Blots as incubated in the secondary antibody (DayLight 800) 1:5000 dilution for 30min at RT with agitation and washed 1X with TBSTT for 15min, 1X with TBST for 15min before scanning with the ODyssey IRD scanner.

Courtesy of Dr. Betty Chung, Dr Pawel Baster, University of Cambridge, United Kingdom

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