DCL1 | Dicer-like protein 1
AS12 2102 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana
|Info:||More information||Product suggestions||Add review|
1 : 1000 with standard ECL (WB)
|Expected | apparent MW||
|Predicted reactivity||Arabidopsis thaliana|
|Not reactive in||
Recommend protein load is from 40-50 µg/well (using 1.5 mm spacers helps to obtain wider wells)
Francisco-Mangilet et al. (2015). THO2, core member of the THO/TREX complex, is required for micro RNA production in Arabidopsis. Plant J. 2015 May 14. doi: 10.1111/tpj.12874.
Proteins from nuclear fraction and cytoplasmic fraction of Arabdiospsis thaliana were separated on 7 % SDS-PAGE using semi-dry transfer and blotted 1h to PVDF. Blots were blocked 2,5% milk in TBS-T for over night at 4 degrees with agitation. Blot was incubated in the primary antibody (1:500; anti-DCL1, AS12 2102, lot 1508 (N) )) in PBS-T for 1h at RT with agitation. The antibody solution was decanted and the blot was washed 3 times for 15 min in PBS-T at RT with agitation. Blot was incubated in secondary antibody ( 1:10000; anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera cat no. AS09 607) for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 360 seconds.
Nuclear fraction: Nuclear fraction preparation was described in article: „The SERRATE protein is involved in alternative splicing in Arabidopsis thaliana” - Raczyńska et al., 2014.
Courtesy of M. Sc. Mateusz Bajczyk, Institute of Molecular Biology and Biotechnology, UAM, Poland.
||| For other applications or usage on species other than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions please use the LiveChat option or contact us at email@example.com