HYL1 | hyponastic leave phenotype ds-RNA binding protein

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AS06 136 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana


81 st
Item No:
AS06 136

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product information

HYL1 is nuclear localized, double stranded RNA-binding protein involved in microRNA (miRNA) biosynthesis. Synonyme: F21M12.9 protein EMBL AAB60726.1


KLH-conjugated synthetic peptide derived from Arabidopsis thaliana Hyl1 protein sequence O04492, At1g09700

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 ĩl
Reconstitution For reconstitution add 50 ĩl of sterile water.

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB), RIP (RNA immunoprecipitation assay)
Related products

collection of antibodies to proteins involved in DNA/RNA metabolism and cell cycle

Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution

1: 1000

Expected | apparent MW

45.5 kDa

Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity

Arabidopsis thaliana

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information
Selected references Francisco-Mangilet et al. (2015). THO2, core member of the THO/TREX complex, is required for micro RNA production in Arabidopsis. Plant J. 2015 May 14. doi: 10.1111/tpj.12874.
Raczynska et al. (2013). The SERRATE protein is involved in alternative splicing in Arabidopsis thaliana. Nucleic Acids Res. Oct 16.
et al. (2012). Fast-Forward Genetics Identifies Plant CPL Phosphatases as Regulators of miRNA Processing Factor HYL1. Cell Nov 9.

application example

western blot using anti-HYL1 antibodies

40 µg of total protein from Arabidopsis thaliana rosette leaves extracted with extraction buffer (100 mM Tris HCl, pH 7.5; 10% glycerol; 5 mM EDTA; 5 mM EGTA; 150 mM NaCl; 0.75% Triton X-100; 0.05% SDS; 1 mM DTT; 1x Complete Mini EDTA-free protease inhibitor (Roche)) were separated on 12 % SDS-PAGE using semi-dry transfer and blotted 1h to PVDF. Blots were blocked with 5% milk in TBS-T O/N at 4°C with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1,5 h at RT with agitation. The antibody solution was decanted and the blot was washed 3 times for 10 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:50 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 60 seconds.  There were no other bands present on this blot in applied conditions.

Courtesy of Dr. Dorota Raczyńska, M.Sc Agata Stepień Adam Mickiewicz University, Poland

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