S6K1-2 | Ribosomal-protein S6 kinase homolog 1,2 - phosphorylated

323 €
Buy 2 items of this product for 238 €/each
Buy 3 items of this product for 213 €/each

AS13 2664  | clonality: polyclonal  |  host: rabbit  |  reactivity: Arabidopsis thaliana


50 st
Item No:
AS13 2664

Info: More information Product suggestions Add review
product information

Ribosomal S6 kinase 1/2 (S6K1/2) involved in TOR signaling pathway, in osmotic stress response. Activated by PDK1 and repressed during osmotic stress. Expressed in all tissues, especially during high metabolic activity in growing buds, root tips, leaf margins and germinating seeds. Alternative names: AtPK1/AtPK6 S6K1), AtPK2/AtPK19 (S6K2).


KLH-conjugated peptide containing phospho-Thr, derived from Arabidopsis thaliana S6K1: UniProt: P42818, TAIR: AT3G08730 and S6K2: UniProt: Q39030, TAIR: AT3G08720. Due to high amino acid homology, chosen peptide is conserved in both proteins:  S6K1 and S6K2.

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum
Format Lyophilized in PBS pH 7.4
Quantity 50 ĩg
Reconstitution For reconstitution add 50 ĩl of sterile water.

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Never store this antibody in 4°C.

Tested applications western blot (WB)
Related products

AS12 1855 | anti-S6K1/2 | Ribosomal S6 kinase 1/2, rabbit antibody
AS12 2608
| anti-TOR | Target of rapamycin, rabbit antibody

Plant protein extraction buffer

Secondary antibodies

Additional information

to be added when available

application information
Recommended dilution

1 : 500 with standard ECL (WB)

Expected | apparent MW

52.6 kDa (S6K1) and 53 kDa (S6K2) | 60 kDa

Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity Thelungiella halophila, for other species - please inquire
Not reactive in

no confirmed exceptions from predicted reactivity are currently known

Additional information

to be added when available

Selected references

to be added when available, antibody released in March 2015

application example

western blot using anti-S6K1-2 | Ribosomal-protein S6 kinase homolog 1,2 - phosphorylated antibodies

30 µg of total protein of Arabidopsis thaliana 10 days old seedlings extracted with Lacus buffer with or without phosphatase inhibitors and kept at 4°C prior to denaturation for 5 min. at 95°C. Samples were separated on 6.5 % SDS-PAGE and blotted 2h to PVDF. Phosphatase treatment was done with CIAP ("+"  phosphatase inhibitors added, "-" phosphatase inhibitors not included). Blots were blocked with 5% milk in TBST for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1:500 for overnight at 4C with agitation. The antibody solution was decanted and the blot was washed three times for 15 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated from Agrisera, AS09 602) diluted to 1:10 000 in 2.5% milk in TBST for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions (PIERCE). Exposure time was few minutes.

Courtesy of Dr. Rossana Henriques, CRAG, Spain

||| For other applications or usage on species other than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions please use the LiveChat option or contact us at