PR-3 | CHN | class I chitinase
AS07 207 | clonality: polyclonal | host: rabbit | reactivity: N. tabacum, P. abies, S. esculentum, S. tuberosum, V. vinifera | cellular marker of vacuolar contents
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8 ug/ml with standard ECL (WB)
|Expected | apparent MW||
35, 34 | 32 and 34 kDa
Nicotiana tabacum, Picea abies, Solanum esculentum, Solanum tuberosum, Vitis vinifera
Arabidopsis thaliana, Nicotiana tabacum, Solanum lycopersicum, Zea mays
|Not reactive in||
to be determined
Important note: For blocking 5 % skim milk in PBS without Ca++ should be used.
This antibody is purified by affinity chromarography on Portein G.
|Selected references||Yi-Ru et al. (2016). Laminarin modulates the chloroplast antioxidant system to enhance abiotic stress tolerance partially through the regulation of the defensin-like gene expression. Plant Science, Volume 247, June 2016, Pages 83–92.
Falcioni et al. (2013). Effect of salicylic acid treatment on tomato plant physiology and tolerance to potato virus X infection. Eur J Plant Pathol DOI 10.1007/s10658-013-0333-1.
Munger et al. (2012). Beneficial 'unintended effects' of cereal cystatin in transgenic lines of potato, Solanum tuberosum. BMC Plant Biology, 12:198.
Sticher et al. (1993). Posttranslational processing of a new class of hydroxyproline-containing proteins: Prolyl hydroxylation and C-terminal cleavage of tobacco (Nicotiana tabacum) vacuolar chitinase. Plant Physiol. 101, 1239-1247.
Detection of tobacco chitinase I in ng loaded per respective well using anti-tobacco chitinase I antibodies. Primary antibodies have been used at 8µg/ml.
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