AS11 1811 | clonality: polyclonal | host: rabbit | reactivity:A.thalina, C. pepo, N. tabacum
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1 : 50 (ICC), (IG)
|Expected | apparent MW|
Arabidopsis thaliana, Cucurbita pepo L. subsp. peop var. styriaca Greb, Nicotiana tabacum cv. samsun
|Not reactive in||
no confirmed exceptions from predicted reactivity are currently known
Immunogold labeling for electron microscopy
Block ultrathin sections (80nm) prepared for immunogold labeling with 2% bovine serum albumine (BSA) in phosphate buffered saline (PBS, pH 7.2). Then treat the sections with the primary antibody (anti-γ-glutamylcysteine rabbit polyclonal IgG) diluted 1:50 in PBS containing 1% BSA for 2 h at room temperature. After a short rinse in PBS (3 X 5 min), incubate samples with a gold-conjugated secondary antibody (goat anti-rabbit IgG; eg. 10nm) diluted 1:50 in PBS including 1% BSA for 90 min at room temperature. After a short wash in PBS (2 X 5 min), and distilled water (3 X 5 min) observe grids under a transmission electron microscope.
Koffler et al. (2011). Subcellular distribution of glutathione precusors in Arabidopsis thaliana. Journal of Integrative Plant Biology. 53: 930-941.
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