GR | Glutathione reductase
AS06 181 | clonality: polyclonal | host: rabbit | reactivity: A.toxicaria, B.rapa, N. tabacum, M. sativa, S. vulgaris, S. tuberosum, Z. mays
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1: 5000 with standard ECL (WB), needs to be optimized, 2 µg (IP), 1: 1000 (IL)
|Expected | apparent MW||
Arabidopsis thaliana, Hordeum vulgare, Nicotiana tabacum, Pisum sativum, Silene vulgaris, Solanum tuberosum, Zea mays, Scenedesmus quadricauda (algae)
dicots including: Brassica rapa, Glycine max;
|Not reactive in||
no confirmed exceptions from predicted reactivity known in the moment
This antibody will recognize the chloroplastic and cytoplasmic forms of the enzyme.
|Selected references||Hattab et al. (2015). Characterisation of lead-induced stress molecular biomarkers in Medicago sativa plants. Environm. Exp. Botany. Volume 123, March 2016, Pages 1–12.
Shaw et al. (2015). β-aminobutyric acid mediated drought stress alleviation in maize (Zea mays L.). Environ Sci Pollut Res Int. 2015 Sep 29.
Sobrino-Plata et al. (2014). Glutathione is a key antioxidant metabolite to cope with mercury and cadmium stress. Plant Soil, DOI 10.1007/s11104-013-2006-4.
Kovácik et al. (2013). Oxidative stress, uptake and bioconversion of 5-fluorouracil in algae. Chemosphere. 2013 Dec 28. pii: S0045-6535(13)01679-2. doi: 10.1016/j.chemosphere.2013.11.074. (immunolocalization in algae)
Sobrino-Plata et al. (2013). Specific stress responses to cadmium, arsenic and mercury appear in the metallophyte Silene vulgaris when grown hydroponically. RSC Advances, Jan 24.
10 µg of total protein from (1) Arabidopsis thaliana leaf extracted with Protein Extration Buffer, PEB (AS08 300), (2) Nicotiana tabaccum leaf extracted with PEB, (3) Zea mays extracted with PEB, (4) Hordeum vulgare leaf extracted with PEB, (5) Physcomitrella patens total cell extracted with PEB, (6) Chlamydomonas reinhardtii total cell extracted with PEB, (7) Synochocystis elongatus total cell extracted with PEB, extracted with PEB, were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to nitrocellulose. Blots were blocked in 2 % low fat dry milk in TBS-T (0.1 % Tween 20) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 2000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Abcam) diluted to 1:30 000 for 1h at room temperature with agitation. The blots were washed as above and developed for 30 seconds with WEST PICO reagent according the manufacturers instructions.
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