GR | Glutathione reductase

350 €

AS06 181  | clonality: polyclonal  |  host: rabbit  |  reactivity: A.toxicaria, B.rapa, N. tabacum, M. sativa, S. vulgaris, S. tuberosum, Z. mays


40 st
Item No:
AS06 181

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product information

Glutathione reductase (GR, EC is an important enzyme for  plant protection against environmental stress. It functions in plant defense reactions in the conversion of glutathione disulphide to reduced glutathione (GSH).


maltose binding protein (MBP) fusion of Zea mays GR, O64409

Host Rabbit
Clonality Polyclonal
Purity Total IgG
Format Lyophilized in PBS pH 7.4
Quantity 100 ĩl
Reconstitution For reconstitution add 100 ĩl of sterile water.

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB), immunolocalization (IL), immunoprecipitation (IP)
Related products

AS06 183 | anti-GS, glutathione synthase antibodies

collection of antibodies to stress proteins

Additional information

total IgG concentration is 7 µg/ µl

application information
Recommended dilution

1: 5000 with standard ECL (WB), needs to be optimized, 2 µg (IP), 1: 1000 (IL)

Expected | apparent MW

54 kDa

Confirmed reactivity

Arabidopsis thaliana, Hordeum vulgare, Nicotiana tabacum, Pisum sativum, Silene vulgaris, Solanum tuberosum, Zea mays, Scenedesmus quadricauda (algae)

Predicted reactivity

dicots including: Brassica rapa, Glycine max;
monocots including: Oryza sativa, trees: Populus balsamifera

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

This antibody will recognize the chloroplastic and cytoplasmic forms of the enzyme.

Selected references Hattab et al. (2015). Characterisation of lead-induced stress molecular biomarkers in Medicago sativa plants. Environm. Exp. Botany. Volume 123, March 2016, Pages 1–12.
Shaw et al. (2015). β-aminobutyric acid mediated drought stress alleviation in maize (Zea mays L.). Environ Sci Pollut Res Int. 2015 Sep 29.
Sobrino-Plata et al. (2014). Glutathione is a key antioxidant metabolite to cope with mercury and cadmium stress. Plant Soil, DOI 10.1007/s11104-013-2006-4.
et al. (2013). Oxidative stress, uptake and bioconversion of 5-fluorouracil in algae. Chemosphere. 2013 Dec 28. pii: S0045-6535(13)01679-2. doi: 10.1016/j.chemosphere.2013.11.074. (immunolocalization in algae)
Sobrino-Plata et al. (2013). Specific stress responses to cadmium, arsenic and mercury appear in the metallophyte Silene vulgaris when grown hydroponically. RSC Advances, Jan 24.

application example

10 µg of total protein from (1) Arabidopsis thaliana leaf extracted with Protein Extration Buffer, PEB (AS08 300), (2) Nicotiana tabaccum leaf extracted with PEB, (3) Zea mays extracted with PEB, (4) Hordeum vulgare leaf extracted with PEB, (5) Physcomitrella patens total cell extracted with PEB, (6) Chlamydomonas reinhardtii total cell extracted with PEB,  (7) Synochocystis elongatus total cell extracted with PEB, extracted with PEB, were separated on  4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to nitrocellulose. Blots were blocked in 2 % low fat dry milk in TBS-T (0.1 % Tween 20) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 2000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Abcam) diluted to 1:30 000 for 1h at room temperature with agitation. The blots were washed as above and developed for  30 seconds  with WEST PICO reagent according the manufacturers instructions.

The nature of 40 kDa cross reaction in this experiment is not known.


western blot using anti-GR antibodies

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