slr1641 | ATP-dependent chaperone clpB

345 €

AS08 344  |  clonality: polyclonal  |  host: rabbit  |  reactivity: cyanobacteria


9 st
Item No:
AS08 344

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product information

ClpB protein is essential for resistance to high temperature stress. It functions to dissolve inactive protein aggregates that accumulate at high temperatures. Giese and Vierling (2002) Changes in oligomerization are essential for the chaperone activity of a small heat shock protein in vivo and in vitro. J Biol Chem; 277(48): 46310-8.


recombinant clpB1 protein, derived from Synechocystis PCC 6803 strain slr1641 sequence; protein has an internal translation site. The nomenclature used is reverse of what is mentioned in the cyanobase.

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 100 ĩl
Reconstitution For reconstitution add 100 ĩl of sterile water

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB)
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Additional information
application information
Recommended dilution

1: 3000 with standard ECL (WB)

Expected | apparent MW

98.1 | 85.4 and 105 | 95 kDa for Synechocystis

Confirmed reactivity

Synechocystis PCC 6803, Solanum lycopersicum

Predicted reactivity cyanobacteria, Francisella sp.
Not reactive in Chlamydomonas reinhardtii
Additional information
Selected references Gonzalez-Esquer and Vermaas (2013). ClpB1 overproduction in Synechocystis sp. strain PCC 6803 increases tolerance to rapid heat shock. Appl Environ Microbiol. 2013 Oct;79(20):6220-7. doi: 10.1128/AEM.01661-13. Epub 2013 Aug 2.

application example

 western blot detection of slr1641 protein 10 μg of total protein from Synechocystis PCC 6803 wild type (+ClpB1) and slr1641 deletion mutant, control (C) and heat shocked samples (HS) was separated on 8% PAA gel and blotted on nitrocellulose membrane . Filters were blocked (1h), incubated with 1: 3000 anti-ClpB1 antibodies (2h) followed by incubation with 1: 2500 secondary anti-rabbit (1h) coupled to HRP and visualization with standard ECL (Amersham).

Courtesy of Dr. Elizabeth Vierling, University of Massachusetts, USA

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