HSP17.6 | cytosolic class I heat shock protein 17.6
AS08 284 | clonality: polyclonal | host: hen | reactivity: Arabidopsis thaliana
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1: 1000 with standard ECL (WB)
|Expected | apparent MW||
|Not reactive in||
no confirmed exceptions from predicted reactivity known at the moment
There are six total class I genes. Essentially this antibody might react to some extent with all of them. But does not react with class II, organelle, or any other shsp classes.
Mao and Sun (2015). Arabidopsis seed-specific vacuolar aquaporins are involved in maintaining seed longevity under the control of ABSCISIC ACID INSENSITIVE 3. J Exp Bot. 2015 May 26. pii: erv244.
10 µg of total protein from (C) Arabidopsis thaliana WT, non-stressed, (HS) Arabidopsis thaliana WT stressed at 38ºC were separated on 15% SDS-PAGE and blotted 1h to nitrocellulose (Biorad). Blots were incubated in the primary antibody at a dilution of 1: 1 000 for 1h at room temperature with agitation and a secondary HRP-conjugated antibody (1: 10 000 from Abcam).
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