HSP101 | ClpB heat shock protein, N-terminal

345 €

AS08 283 | clonality: polyclonal | host: hen | reactivity: Arabidopsis thaliana


15 st
Item No:
AS08 283

Info: More information Product suggestions Add review
product information

Hsp101/ClpB is a member of HSP100 protein family. These proteins help protein aggregates formed during heat stress to fall apart to allow them to be refolded by other chaperones. In spite of expression during heat stress members of HSP100 protein family are also expressed during seed development.


recombinant Arabidopsis thaliana Hsp101/ClpB, N-terminal 145 amino acids P42730, At1g74310

Host Hen
Clonality Polyclonal
Purity Total IgY
Format Liquid in PBS pH 8.0, 0.02% sodium azide
Quantity 200 ĩl

store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

AS07 253 | anti-HSP101 | ClpB heat shock protein, N-terminal

AS08 290 | anti-HSP101 | nuclear/cytoplasmatic heat shock protein, rabbit antibody with reactivity to Zea mays

collection of antibodies to plant heat shock proteins

Additional information

to be added when available

application information
Recommended dilution

1: 1000 with standard ECL (WB)

Expected | apparent MW

101 kDa

Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity

dicots including: Glycine max, Nicotiana tabacum, Vitis vinifera

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

to be added when available

Selected references

to be added when available

application example

western blot using anti-Hsp101/ClpB antibodies

2 µg of total protein from (1) Arabidopsis thaliana WT stressed at 22ºC, (2) Arabidopsis thaliana WT stressed at 38ºC for 1.5 hour followed by a transfer to 22° for 2 hours, (3) Arabidopsis thaliana Hsp101 null mutnat grown at 22°C, (4) Arabidopsis thaliana Hsp101 null mutant stressed at 38ºC for 1.5 hour followed by a transfer to 22ºC for 2 hours were separated on 8 % SDS-PAGE and blotted 1h to nitrocellulose (Biorad). Blots were incubated in the primary antibody at a dilution of 1: 1 000 for 1h at room temperature with agitation and a secondary HRP-conjugated antibody (1: 10 000 from Abcam).

||| For other applications or usage on species other than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions please use the LiveChat option or contact us at