HSP101 | ClpB heat shock protein, N-terminal

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AS07 253 | clonality: polyclonal | host: rabbit | reactivity: A.thaliana, A. tequilana var. Weber, N. tabacum, S. lycopersicum, T. salsuginea


35 st
Item No:
AS07 253

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product information

Hsp101/ClpB is a member of HSP100 protein family. These proteins help protein aggregates formed during heat stress to fall apart to allow them to be refolded by other chaperones. HSP101 is a cytosolic heat shock protein required for acclimation to high temperature.


Recombinant Hsp101 N-terminal derived from the sequence of Arabidopsis thaliana Hsp101 protein P42730, At1g74310

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
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collection of antibodies to plant heat shock proteins

Additional information

to be added when available

application information
Recommended dilution

1: 1000 with standard ECL (WB)

Expected | apparent MW

101 kDa

Confirmed reactivity

Arabidopsis thaliana, Agave tequilana, Solanum lycopersicum, Thellungiella salsuginea

Predicted reactivity

dicots including: Glycine max, Vitis vinifera, monocots including: Oryza sativa

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

to be added when available

Selected references Muench et al. (2016). Reactive electrophilic oxylipins trigger a heat stress-like response through HSFA1 transcription factors. J of Exp. Botany,10.1093/jxb/erw376.
Fragkostefanakis et al. (2016). HsfA2 controls the activity of developmentally and stress-regulated heat stress protection mechanisms in tomato male reproductive tissues. Plant Physiol. 2016 Feb 25. pii: pp.01913.2015.
Mishra et al. (2015). Characterization of 5'UTR of rice ClpB-C/Hsp100 gene: evidence of its involvement in post-transcriptional regulation. Cell Stress Chaperones. 2015 Nov 6.
Kumar et al. (2015). Expression analysis of ClpB/Hsp100 gene in faba bean (Vicia faba L.) plants in response to heat stress. Saudi Journal of Biol. Sciences, March 2015.
Almoguera et al. (2015). Heat shock transcription factors involved in seed desiccation tolerance and longevity retard vegetative senescence in transgenic tobacco. Planta. 2015 May 29.
Yamauchi et al. (2015). Reactive short-chain leaf volatiles act as powerful inducers of abiotic stress-related gene expression. Sci Rep. 2015 Jan 26;5:8030. doi: 10.1038/srep08030.
Mishra and Grover (2014). Intergenic Sequence between Arabidopsis Caseinolytic Protease B-Cytoplasmic/Heat Shock Protein100 and Choline Kinase Genes Functions as a Heat-Inducible Bidirectional Promoter 1. Plant Physiol. 2014 Nov;166(3):1646-58. doi: 10.1104/pp.114.250787.
Gamburg et al. (2014). The Relationship between the Differences in Frost Resistance of Arabidopsis and Thellungiella and Heat Shock Proteins and Dehydrins. Russian J. Plant Physiol. May 2014, Volume 61, Issue 3, pp 318-323
et al. (2014). Mitochondrial Retrograde Regulation of HSP101 Expression in Arabidopsis thaliana under Heat Stress and Amiodarone Action. Russian J. Plant Physiol. 61 (1):88-98. (Western blot, cell culture)

application example



2 µg of total protein from (1) Arabidopsis thaliana WT stressed at 38ºC for 1.5 hour, (2) Arabidopsis thaliana HSP101 null mutant (hot 1-3) were separated on 7.5% SDS-PAGE and blotted 1h to nitrocellulose (Biorad). Blots were incubated in the primary antibody at a dilution of 1: 1 000 for 1h at room temperature with agitation and secondary HRP-conjugated antibody (1: 10 000 from Abcam).


western blot using anti-HSP101 antibodies

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