HSP16.6 | cytosolic class I heat shock protein 16.6
AS08 286 | clonality: polyclonal | host: rabbit | reactivity: Synechocystis sp. PCC 6803
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1: 5000 with standard ECL (WB)
|Expected | apparent MW||
Synechocystis sp. PCC 6803
Synechococcus sp., Thermosynechococcus sp.
|Not reactive in||
no confirmed exceptions from predicted reactivity known in the moment
on a gradient gel Hsp16.6 runs closer to 11 kDa
Gunnelius et al. (2014). The omega subunit of the RNA polymerase core directs transcription efficiency in cyanobacteria. Nucleic Acids Res. 2014 Jan 29.
Left panel: purified recombinant Hsp16.6 in 0.5, 1 and 2 ng per well. Right panel: 5 µg of total protein from Synechocystis PCC 6803 null mutant and WT were separated on 9-17 % SDS-PAGE and blotted 1h to nitrocellulose (Biorad). Blots were incubated in the primary antibody at a dilution of 1: 5 000 for 1h at room temperature with agitation and a secondary HRP-conjugated antibody (1: 10 000 from Abcam).
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