HSP16.9 | 16.9 kDa class I heat shock protein 2

345 €

AS12 2570 | clonality: polyclonal | host: rabbit | reactivity: Triticum aestivum


30 st
Item No:
AS12 2570

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product information

Hsp16.9 belongs to a family of class I of a small heat shock proteins (HSP20) family. Localized in cytoplasm. Alternative protein name: Heat shock protein 16.9B


Recombinant protein of Triticum aestivum Q41560

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 100 ĩl
Reconstitution For reconstitution add 100 ĩl of sterile water.

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

collection of antibodies to heat shock proteins

Additional information
application information
Recommended dilution

1: 1000 - 1:10 000 with standard ECL (WB)

Expected | apparent MW

16.8 kDa

Confirmed reactivity

Triticum aestivum

Predicted reactivity Aegilops kotschyi, Triticum sp., Hordeum vulgare
Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information
Selected references

to be added when available, antibody released in June 2013.

application example

western blot using anti-HSP16B antibodies

From 0.1-200 ng of recombinant HSP16B protein from Triticum aestivum were separated on 15 % SDS-PAGE and blotted 1h to nitrocellulose using semi-dry transfer at 15 V for 1 hour in a buffer containing: 48mM Tris, 39 mM Glycine, 0.02% SDS, 20 % methanol, pH 9.2. Blots were blocked with with 2.5% milk in TBS (50mM Tris, 150 mM NaCl, pH 7.5) for 1 hour at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 10 000 for 2h at RT TBS with 2.5% milk with agitation. Three washes (15 minutes each) were carried out with TBS and 0.05% Tween20 at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, diluted to 1:10 000 in  for 1h at RT with agitation. The blot was washed as above and developed using Pierce western blotting substrate (#32106). The exposure time used for visualization was 6.5 minutes.

Courtesy of Indu Santhanagopalan, Dept. of Biochem and Mol Biology, Lederle Graduate Research Center, USA.

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