HSP17.6 | Cytosolic class I heat shock protein 17.6

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AS07 254 | clonality: polyclonal | host: rabbit | reactivity: A. thaliana, A. tequilana, C. sativus, I. pumilla (perennial monocot), P. ternata,  P. sylvestris, S. vulgaris


24 st
Item No:
AS07 254

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product information

Hsp17.6 belongs to a family of class I of a small heat shock proteins. They are induced once a plant cells are stressed by an increased temperature. The way small hsp proteins are protecting a living cell are not fully understood. They seem to be involved in chaperone functions by protecting other proteins from irreversible denaturation. Small hsp function also in a late seed maturation process.


Recombinant protein. Arabidopsis thaliana Hsp17.6 CI (class one) P13853, At1g53540

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 ĩl
Reconstitution For reconstitution add 50 ĩl of sterile water.

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

AS08 284 | anti-HSP17.6 | cytosolic class I heat shock protein 17.6 hen antibody

AS07 255 | anti-HSP17.7 | cytosolic class II heat shock protein 17.7

collection of antibodies to plant heat shock proteins

Additional information
application information
Recommended dilution

1: 1000 with standard ECL (WB)

Expected | apparent MW

17.6 kDa

Confirmed reactivity

Arabidopsis thaliana, Agave tequilana var. Weber, Cucumis sativus, Iris pumilla (perennial monocot), Pinellia ternata, Pinus sylvestris, Silene vulgaris

Predicted reactivity higher plants
Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information There are six total class I genes. Essentially this antibody might react to some extent with all of them. But does not react with class II, organelle, or any other shsp classes.
Selected references Yamauchi et al. (2015). Reactive short-chain leaf volatiles act as powerful inducers of abiotic stress-related gene expression.
Pyatrikas et al. (2014). Mitochondrial Retrograde Regulation of HSP101 Expression in Arabidopsis thaliana under Heat Stress and Amiodarone Action. Russian J. Plant Physiol. 61 (1):88-98. (Western blot, cell culture)
Florentin et al. (2013). Stress induces plant somatic cells to acquire some features of stem cells accompanied by selective chromatin reorganization. Dev. Dyn. Oct; 242(10):1121-33.

application example

western blot using anti-Hsp17.6 antibodies

15 µg of total protein from (HS) heat shocked Arabidopsis thaliana , (C) Arabidopsis thaliana control plants, (1,2,5,10) 1,2,5,10 ng of recombinant puridfied HSP17.6 were separated on 15%SDS-PAGE and blotted 1h to nitrocellulose(Biorad). Blots were incubated in the primary antibody at a dilution of 1: 1000 for 1h at room temperature with agitation and secondary HRP-conjugated antibody (1: 10 000 from Abcam).

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