SAL1 | Sal1 phosphatase

345 €

AS07 256  |  clonality: polyclonal  |  host: rabbit   | A.thaliana, P. tremula, L. esculentum, G. max, N. tabaccum


9 st
Item No:
AS07 256

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product information

The plant SAL1 (known also as FIERY1, FRY1, HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENES 2, HOS2, MBM17.8, MBM17_8) is a 353 aa protein homologous to the HAL2 and CysQ phosphatases of yeast and Escherichia coli, respectively. The SAL1 protein expressed in E. coli shows nucleotidase and inositol phosphatase activities. SAL1 is proposed to participate in the sulfur assimilation pathway as well as in the phosphoinositide signaling pathway.


Recombinant SAL1, full-length protein, 353 amino acids. The cDNA of SAL1 (At5g63980, protein Q42546) was cloned into pHUE expression vector and the protein has been produced and purified according to Baker et al 2005 

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 100 ĩl
Reconstitution For reconstitution add 100 ĩl of sterile water.

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

collection of antibodies to plant stress proteins

Additional information

to be added when available

application information
Recommended dilution

1: 1000 with ECL (WB)

Expected | apparent MW

37.5 | 41 kDa (Arabidopsis thaliana)

Confirmed reactivity

A.thaliana, P. tremula,L. esculentum, G. max , N. tabaccum

Predicted reactivity

G. hirsutum, O. sativa

Not reactive in

Ch. reinhardtii

Additional information

to be added when available

Selected references

to be added when available

application example

7.5 µg of total leaf protein extracted with PEB (AS08 300) from (1) Nicotiana tabacum, (2) Glycinia max, (3) Lycopersicon esculentum, (4) Chlamydomonas reinhardtii, (5) Populus tremula and (6) Arabidopsis thaliana were separated on  4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to nitrocellulose. Filters were blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-SAL1 (AS07 256, 1:1000, 1h) and secondary anti-rabbit (1:20000, 1 h) HRP-conjugated antibody (Abcam) in TBS-T containing 2% low fat milk powder.  Antibody incubations were followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signal was detected with standard ECL (GE Healthcare) using a Fuji LAS-3000 CCD (240s, standard sensitivity).


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